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Anti f4 80 percp

Manufactured by Thermo Fisher Scientific
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The Anti-F4/80-Percp is a laboratory reagent used for the detection and identification of F4/80-positive cells, such as macrophages, in flow cytometric analysis. It is a monoclonal antibody conjugated with the PerCP (Peridinin Chlorophyll Protein) fluorescent dye.

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Lab products found in correlation

Anti cd11β fitc, by Thermo Fisher Scientific (2 mentions) Anti ly6g percp pe, by Thermo Fisher Scientific (2 mentions) Anti cd45 apc, by Thermo Fisher Scientific (2 mentions) Facscalibur, by BD (2 mentions)

Close spelling variants of this product

F4/80 (353 citations) Anti-F4/80 (122 citations) F4/80‐PERCP (2 citations) Anti-F4/80-PerCP-Cyanine5.5 (2 citations) Percp-Cy5.5-conjugated anti-mouse F4/80 (2 citations)

2 protocols using anti f4 80 percp

1

Microglial Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated microglial cells from brain tissue and BV2 cells were washed in PBS twice. The cells were blocked with CD16/32 for 10 min and then stained for 30 min in a dark chamber with appropriate fluorochrome-conjugated antibodies (Anti-CD45-APC, eBioscience, Anti-CD11β-FITC, eBioscience, Anti-F4/80-Percp, eBioscience, Anti-Ly6G-Percp-PE, eBioscience). After washing thrice in PBS, flow cytometry was performed to analyze microglial cell populations using a BD FACSCalibur flow cytometer (BD Bioscience, San Jose, CA) and FlowJo software (Tree Star Inc., Ashland, OR).
Xu F., Mu J., Teng Y., Zhang X., Sundaram K., Sriwastva M.K., Kumar A., Lei C., Zhang L., Liu Q.M., Yan J., McClain C.J., Merchant M.L, & Zhang H.G. (2021). Restoring Oat Nanoparticles Mediated Brain Memory Function of Mice Fed Alcohol by Sorting Inflammatory Dectin-1 Complex Into Microglial Exosomes. Small (Weinheim an der Bergstrasse, Germany), 18(6), e2105385.
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2

Microglial Cell Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated microglial cells from brain tissue and BV2 cells were washed in PBS twice. The cells were blocked with CD16/32 for 10 min and then stained for 30 min in a dark chamber with appropriate fluorochrome-conjugated antibodies (Anti-CD45-APC, eBioscience, Anti-CD11β-FITC, eBioscience, Anti-F4/80-Percp, eBioscience, Anti-Ly6G-Percp-PE, eBioscience). After washing thrice in PBS, flow cytometry was performed to analyze microglial cell populations using a BD FACSCalibur flow cytometer (BD Bioscience, San Jose, CA) and FlowJo software (Tree Star Inc., Ashland, OR).
Xu F., Mu J., Teng Y., Zhang X., Sundaram K., Sriwastva M.K., Kumar A., Lei C., Zhang L., Liu Q.M., Yan J., McClain C.J., Merchant M.L, & Zhang H.G. (2021). Restoring Oat Nanoparticles Mediated Brain Memory Function of Mice Fed Alcohol by Sorting Inflammatory Dectin-1 Complex Into Microglial Exosomes. Small (Weinheim an der Bergstrasse, Germany), 18(6), e2105385.
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