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Sykfl fl

Manufactured by Jackson ImmunoResearch

Sykfl/fl is a laboratory product used for research purposes. It functions as a genetic tool to manipulate target genes in cells. The product details and intended use should be obtained from the manufacturer or other reliable sources.

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2 protocols using sykfl fl

1

Mice Strains for Immunology Research

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AIDCre/+, UBC-CreERT2, SJL CD45.1+, PAGFP, GFP, MD4, OT-II, DsRed-expressing mice, Sykfl/fl, and Rosa26fl-stop-fl KrasG12D mice were purchased from the Jackson Laboratory. NP-specific B1-8hi mice (Shih et al., 2002b (link)) were a gift from M. Nussenzweig (The Rockefeller University, New York, NY). SykY317F mice were generated as described below. WT mice (C57BL/6) were provided by Harlan. All experiments using mice were approved by the Weizmann Institute Animal Care and Use Committee.
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2

Murine Hematopoietic Cell Lineage Tracing

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C57BL/6J (stock #00664), PF4-iCre (stock #008535), Sykfl/fl (stock # 017309) and ROSA26iDTR (stock #007900) were purchased from the Jackson Laboratory (JAX). C57BL/6-Ly5.1 (CD45.1, stock #564) was purchased from Charles River Laboratories. Mice were allowed to adjust to housing in the MSKCC vivarium for one week. Age-matched, sex-matched, and/or littermate controls were used for experiments as appropriate. Animals were euthanized by either pentobarbital injection or CO2 per IACUC guidelines.
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