Beyoclick edu 488 imaging kit

The effect of Tan IIA on the proliferative activity of hBMSCs was evaluated using BeyoClick™ EDU 488 imaging Kit (Beyotime, China). Cells were seeded onto 96-well plates, treated with Tan IIA (0.1 1, and 10 μM) for 6, 12, 24, 36 or 48 h or Tan IIA (1 μM) plus Debio-1347 (0.1, 0.5, 1 or 5 μM) for 24 h, respectively. After that, 20 μM Edu was added to each well, incubated for 2 h at 37 °C, fixed with 4% paraformaldehyde, and permeabilized with 0.3% Triton X-100 for 15 min. Cells were washed with 3% BSA, and then incubated in click reaction solution in the dark for another 30 min. Finally, the absorbance value was measured at a maximum excitation wavelength of 495 nm with maximum emission wavelength of 519 nm.