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Glutathione peroxidase 1 gpx 1

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Glutathione peroxidase 1 (GPx-1) is an enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and corresponding alcohols, respectively, using glutathione as a reducing agent. It plays a crucial role in protecting cells from oxidative damage by removing reactive oxygen species.

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Lab products found in correlation

Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (2 mentions) Tris hcl gel, by Bio-Rad (1 mentions) Odyssey imaging system, by LI COR (1 mentions) 3 mst, by Novus Biologicals (1 mentions) P47phox, by Santa Cruz Biotechnology (1 mentions) P22phox, by Santa Cruz Biotechnology (1 mentions) P67phox, by Santa Cruz Biotechnology (1 mentions) Nitrotyrosine, by Santa Cruz Biotechnology (1 mentions) Gel doc 2000 imager, by Bio-Rad (1 mentions) Heme oxygenase 1 ho 1, by Santa Cruz Biotechnology (1 mentions) Histone h1, by Abcam (1 mentions)

Spelling variants of this product

GPx-1 (8 citations) Glutathione peroxidase (GPx) (4 citations)

2 protocols using glutathione peroxidase 1 gpx 1

1

Western Blot Analysis of Myocardial Proteins

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Whole cell lysates were prepared using myocardial tissue obtained from mice treated with vehicle or zofenopril at 8 hours. Equal amounts of protein (30 μg), determined by using the BSA Protein Assay, were separated on Tris‐HCl gel (4–20%, Bio‐Rad) and transferred to nitrocellulose membranes.42 The membranes were blocked and then probed with the following primary antibodies overnight at 4°C: CBS (Santa Cruz), CSE (CTH; Abnova), 3‐MST (Novus), eNOS (BD Biosciences), phospho‐eNOS 1177 (p‐eNOS1177; Abcam), phospho‐eNOS 495 (p‐eNOSThr495; Cell Signaling Technology), glutathione peroxidase 1 (GPx‐1; Santa Cruz), thioredoxin 1 (Trx‐1; Santa Cruz), Cu,Zn‐superoxide dismutase (SOD‐1; Santa Cruz), and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH; Santa Cruz). Immunoblots were probed with the appropriate fluorescence conjugate secondary antibodies for 2 hours at room temperature and visualized with the Odyssey Imaging System (LI‐COR Biotechnology), then quantified by using Image J software.
Donnarumma E., Ali M.J., Rushing A.M., Scarborough A.L., Bradley J.M., Organ C.L., Islam K.N., Polhemus D.J., Evangelista S., Cirino G., Jenkins J.S., Patel R.A., Lefer D.J, & Goodchild T.T. (2016). Zofenopril Protects Against Myocardial Ischemia–Reperfusion Injury by Increasing Nitric Oxide and Hydrogen Sulfide Bioavailability. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(7), e003531.
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2

Western Blot Analysis of Oxidative Stress Markers

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Membranes were incubated with primary antibodies against Nrf2 (Abcam, Cambridge,
MA, USA), nitrotyrosine (Santa Cruz Biotechnology), quinone oxidoreductase 1
(NQO1, Santa Cruz), heme oxygenase-1 (HO-1, Santa Cruz), P22phox(Santa Cruz), P47phox (Santa Cruz), P67phox (Santa Cruz),
glutathione peroxidase 1 (GPx-1, Santa Cruz), glyceraldehyde 3-phosphate
dehydrogenase (GAPDH, Santa Cruz), and Histone H1 (Abcam). Blotted membranes
were washed and incubated with secondary antibodies (ZSGB-Bio, Beijing, China)
for 2 hours at room temperature. Protein levels were normalized to levels of
GAPDH or Histone H1. Blots were visualized using a Gel Doc 2000 Imager (Bio-Rad,
Hercules, CA, USA).
Wang D., Hou J., Wan J., Yang Y., Liu S., Li X., Li W., Dai X., Zhou P., Liu W, & Wang P. (2021). Dietary chlorogenic acid ameliorates oxidative stress and improves endothelial function in diabetic mice via Nrf2 activation. The Journal of International Medical Research, 49(1), 0300060520985363.
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