Acetazolamide acz

Acetazolamide (ACZ) and polyethylene glycol (PEG8000) were purchased from Sigma–Aldrich (Madrid, Spain). Dimethylsulfoxide (DMSO), sodium acetate, sodium hydroxide, and dichloromethane (DCM) were obtained from Panreac (Madrid, Spain). Acetonitrile and glacial acetic acid were purchased from Lab-Scan (Gliwice, Poland). Propylene glycol (PG) was purchased from Abaran Materias Primas S.L. (Madrid, Spain). Ultrapure water was produced in the laboratory by a Milli-Q Water Purification System (Millipore Iberica SA, Madrid, Spain).

All used chemicals, solvents, and reagents were purchased and obtained from authorized sources and of analytical grade. Acetazolamide (ACZ) was purchased from Sigma-Aldrich Company, St. Louis, MO, USA. Carvedilol (CAR) was purchased from Hetero Drugs Company, Telangana, India, and supplied by Multipharma Pharmaceutical Company, Heliopolis, Cairo, Egypt. Polyoxyethylene (20) sorbitan monolaurate (Tween^® 20) was obtained from Nice Chemicals Private Limited Company, Kerala, India. Polyethylene glycol sorbitan monostearate (Tween^® 60) was obtained from Alpha Chemika Company, Mumbai, India. Sorbitan monolaurate (Span^® 20) and sorbitan monostearate (Span^® 60) were purchased from Oxford Laboratories Pvt. Ltd. Pharmaceutical Company, Mumbai, India. Cholesterol (95%), methanol, sodium acetate, and acetic acid were purchased from Sigma-Aldrich Company, St. Louis, MO, USA. Double distilled water and deionized water were generated through the spectra/pore dialysis membrane 12,000–14,000 molecular weight cut off (Spectrum Laboratories Inc., Rancho Dominguez, CA, USA) and cellulose nitrate membrane, a Millipore^® Filter 0.45 µm (Sartorius Stedim Biotech GmbH, Goettingen, Germany), hydroxypropylmethylcellulose (HPMC-E4) was obtained from (Tama, Tokyo, Japan); Carbopol was obtained from (Sigma Chemical Co., St. Louis, MO, USA); and have been used throughout the study.