Bovine serum albumin (BSA) was obtained from Fisher Scientific (Pittsburgh, PA, USA). Anhydrous N,N-dimethylformamide (DMF) was from Acros Organics (Morris Plains, NJ, USA). The Bicinchoninic acid (BCA) protein assay Kit, N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) were obtained from Pierce (Rockford, IL, USA). Keyhole limpet hemocyanin (KLH), HAT medium supplement, Freund's adjuvant complete (FAC) and Freund's adjuvant incomplete (FAI) were purchased from Sigma–Aldrich (St. Louis, MO, USA). RPMI-1640 medium with L-glutamine and fetal bovine serum (FBS) were from Hyclone (Logan, UT, USA). Goat anti-mouse immunoglobulin G secondary antibody conjugated with horseradish peroxidase (GAM IgG-HRPO) and tissue culture plates (Costar, Corning) were purchased from Fisher Scientific (Pittsburgh, PA, USA). The 1-pyrene butyric acid (PBA) was obtained from Acros (NJ, USA). All other reagents were of analytical grade and purchased from Fisher Scientific (Pittsburgh, PA, USA).
Balb/c mice were bred at our own facility with the original mice purchased from The Jackson Laboratory, (Bar Harbor, ME, USA) All animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of the College of William and Mary.
Balb/c mice were bred at our own facility with the original mice purchased from The Jackson Laboratory, (Bar Harbor, ME, USA) All animal protocols were reviewed and approved by the Institutional Animal Care and Use Committee of the College of William and Mary.