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Ret 2 iso

Manufactured by Physitemp

The RET-2-ISO is a lab equipment product from Physitemp. It is a precision temperature control device designed for research and laboratory applications. The device regulates temperature with high accuracy and stability, but a detailed description of its core function is not available at this time.

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3 protocols using ret 2 iso

1

Operant Conditioning Chambers for Behavioral Studies

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Operant conditioning chambers (ENV-307A-CT, MedAssociates, St. Albans, VT) were kept in ventilated, sound-attenuating boxes. Each chamber contained a house light, a recessed 2.2 cm-diameter hole for reinforcer presentation on one wall, and three identical holes horizontally arranged and spaced 5.5 cm apart on the opposite wall. The center of each hole was 1.6 cm from the floor. Only the center hole was illuminated in the current study. When the center hole was illuminated, disruption of a photobeam in the hole resulted in access to 0.01 cc of 50% v/v condensed milk/water through the hole on the opposite wall. The operant conditioning chambers were connected to a computer through an interface (MED-SYST-8, MedAssociates). Med-PC software (MedAssociates) controlled experimental events and provided a record of responses. Rectal temperature was measured with a digital thermometer (BAT7001H, Physitemp, Clifton, NJ) attached to a rectal probe designed for mice. The probe was 2 cm long and had a diameter 0.7112 mm along its entire length except for the tip, which was 1.651 mm in diameter (RET-2-ISO, Physitemp, Clifton, NJ). The probe was inserted 2 cm into the rectum.
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2

Operant Conditioning and Milk Reinforcement in Rodents

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Operant conditioning chambers (ENV-307A-CT; Med Associates, St Albans, Vermont, USA) were kept in ventilated and sound-attenuating boxes. These chambers had a house light, a recessed 2.2 cm-diameter hole for the presentation of a liquid reinforcer on one wall, and three holes identical to one another on the opposite wall arranged horizontally and spaced 5.5 cm apart. The center of each hole was placed 1.6 cm from the floor. Only the center hole was illuminated, where a nose-poke into this hole disrupted a photobeam. Disruptions of this photobeam would result in the presentation of 0.01 ml of 50% v/v condensed milk/water through the recessed hole on the opposite wall. Through an interface (MED-SYST-8; Med Associates), the operant conditioning chambers were connected to a computer, and Med-PC software (Med Associates) controlled the experimental events and recorded responses. Rectal temperature was measured with a digital thermometer (BAT7001H; Physitemp, Clifton, New Jersey, USA). The rectal probe was 2-cm long and 0.7112 mm in diameter, except for the tip, which was 1.651 mm in diameter (RET-2-ISO; Physitemp).
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3

Operant Conditioning for Behavioral Assessment

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Operant conditioning chambers (ENV‐307A‐CT; Med Associates) were kept in sound‐attenuating and ventilated boxes. Each chamber contained a light and a recessed 2.2‐cm‐diameter hole on one wall through which reinforcers could be presented. On the opposite wall were three identical holes arranged horizontally, spaced 5.5 cm apart. The centers of each hole were 1.6 cm from the floor of the chamber. During experimental sessions, when the middle hole was illuminated, a mouse could disrupt a photobeam by a nose poke, resulting in the presentation of a 0.01 ml of 50% v/v unsweetened condensed milk/water through the hole on the opposite wall. A computer was connected to the operant conditioning chambers through an interface (MED‐SYS‐8; Med Associates), and experimental sessions were controlled, and responses were recorded, using Med‐PC software (Med Associates). Rectal temperature was recorded using a rectal probe designed for mice attached to a digital thermometer (BAT7001H; Physitemp). The probe length was 2 cm. The probe diameter was 0.7112 mm, except for the tip, which was 1.651 mm (RET‐2‐ISO; Physitemp). The probe was inserted 2 cm into the rectum.
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