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Horseradish peroxidase conjugated anti mouse and anti rabbit immunoglobulin g igg antibody

Manufactured by Merck Group

Horseradish peroxidase-conjugated anti-mouse and anti-rabbit immunoglobulin G (IgG) antibody is a laboratory reagent used for immunodetection techniques. It consists of an antibody specific to mouse or rabbit IgG that is conjugated to the enzyme horseradish peroxidase. This conjugate can be used to detect and visualize target proteins in various immunoassays.

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2 protocols using horseradish peroxidase conjugated anti mouse and anti rabbit immunoglobulin g igg antibody

1

Western Blotting Antibody Panel for Akt Signaling

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Anti-Akt1, anti-Akt2, anti-Akt3, anti-phospho-Akt S473 (pAkt), anti-phospho-Akt1 S473 (pAkt1), anti-phospho-Akt2 S474 (pAkt2), anti-Bax, anti-p53, anti-PUMA, anti-IGF1Rβ, anti-pGSK3β, anti-GSK3β, anti-p21, anti-PRAS40, anti-pPRAS40, anti-HA and anti-active caspase 3 antibodies were obtained from Cell Signaling Technology. Anti-β-actin antibody was purchased from Sigma-Aldrich. Anti-p85 polyclonal antibody was generated in-house and has been described (51 (link)). Horseradish peroxidase-conjugated anti-mouse and anti-rabbit immunoglobulin G (IgG) antibody were purchased from Chemicon.
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2

Antibody Sourcing and Validation

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All primary antibodies in this study except p85 were obtained from Cell Signaling Technology. Anti-p85 polyclonal antibody was generated in-house and has been described (21 (link)). Horseradish peroxidase-conjugated anti-mouse and anti-rabbit immunoglobulin G (IgG) antibody were purchased from Chemicon.
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