HEK293T cells were washed and surface stained at 4 °C with primary antibodies for SERINC5 and cell viability dye Zombie UV (Biolegend). For intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with CytoPerm buffer (Biolegend). Primary antibody anti-HA.11 epitope tag-PE (16B12, Biolegend) was used to detect SERINC5 and IFITMs. Data were acquired on a BD LSR Fortessa X-20 or Calibur cytometer and analyzed using FlowJo.
Anti ha 11 epitope tag pe 16b12
Manufactured by BioLegend
Anti-HA.11 epitope tag-PE (16B12) is a fluorescently-labeled antibody that specifically binds to the HA.11 epitope tag. This product can be used for detection and purification of HA-tagged proteins.
Automatically generated - may contain errors
2 protocols using anti ha 11 epitope tag pe 16b12
1
Surface and Intracellular Staining of SERINC5
2
Surface and Intracellular Staining of SERINC5
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