14c caffeine

Manufactured by American Radiolabeled Chemicals

Sourced in United States, Senegal

14C-caffeine is a radioisotope-labeled compound used in research applications. It contains the radioactive carbon-14 isotope, which allows for the tracking and quantification of caffeine and its metabolites in various experimental settings.

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Lab products found in correlation

2 protocols using 14c caffeine

Radioactive Probes for Cellular Assays

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All radioactive probes (³H-diazepam, ¹⁴C-caffeine, ¹⁴C-sucrose, ³H-digoxin, ³H-oleic acid, ³H-2DG, and ¹⁴C-(L)-alanine) were purchased from American Radiolabeled Chemicals (Saint Louis, MO, USA). Solvable and Ultima Gold scintillation cocktail were obtained from Perkin Elmer (Waltham, MA, USA). Pierce™ BCA Protein Assay Kit, sequencing grade trypsin, TMTpro™ 16plex Label Reagent Set and other reagents used for proteomics were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A mouse Glut1 ELISA kit was sourced from Wuxi Donglin Sci & Tech Development Co., Ltd. (Wuxi, China). Dulbecco’s Modified Eagle Medium (DMEM), bovine serum albumin (BSA) and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Pan Y., Kagawa Y., Sun J., Turner B.J., Huang C., Shah A.D., Schittenhelm R.B, & Nicolazzo J.A. (2022). Altered Blood–Brain Barrier Dynamics in the C9orf72 Hexanucleotide Repeat Expansion Mouse Model of Amyotrophic Lateral Sclerosis. Pharmaceutics, 14(12), 2803.

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Radiolabeled Compound Characterization Protocol

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¹⁴C-mannitol, ¹⁴C-caffeine, ³H-Digoxin and ³H-diazepam were obtained from American Radiolabeled Chemicals, Inc (Saint Louis, MO). Ultima Gold™ liquid scintillation cocktail was purchased from Perkin Elmer (Boston, MA). Valsartan was purchased from Sapphire Bioscience (Redfern, New South Wales, Australia). Digoxin, diazepam and ammonium acetate were obtained from Sigma-Aldrich Pty Ltd (Castle Hill, New South Wales, Australia). HPLC or LC-MS grade acetonitrile and methanol were all purchased from Merck KGaA (Darmstadt, Germany).

Jin L., Pan Y., Tran N.L., Polychronopoulos L.N., Warrier A., Brouwer K.L, & Nicolazzo J.A. (2020). Intestinal permeability and oral absorption of selected drugs are reduced in a mouse model of familial Alzheimer’s disease. Molecular pharmaceutics, 17(5), 1527-1537.

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