The transfersomes were generated using thin-film hydration followed by extrusion. The formulation is presented in
V 100
Manufactured by Büchi
Sourced in Switzerland
The V-100 is a rotary evaporator designed for efficient solvent removal. It features a motorized lift mechanism, a digital display, and a variety of accessories to accommodate different laboratory needs. The V-100 is a versatile and reliable tool for researchers and scientists working in various fields.
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Lab products found in correlation
4 protocols using v 100
1
Transfersomal Delivery of rhEGF
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The transfersomes were generated using thin-film hydration followed by extrusion. The formulation is presented inTable 1 . First, the phospholipid, edge activator, and antioxidant (butylated hydroxytoluene) were dissolved in ethanol and placed in a round-bottom flask. The solution then was evaporated using a rotary vacuum evaporator (Buchi V-100, Switzerland) at 150 rpm and 40°C under vacuum. After the thin layer was formed, it was streamed with nitrogen gas and stored in the refrigerator overnight to allow complete evaporation of the solvent. rhEGF was incorporated along with 0.2 M phosphate buffer solution (pH 7.2) in the hydration process. Hydration of dry lipid film was performed at 50–250 rpm and 37°C for 45 min. The resulting transfersome suspension was extruded 11 times through polycarbonate membranes (200 nm).18 (link)
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The transfersomes were generated using thin-film hydration followed by extrusion. The formulation is presented in
2
Vacuum Drying of Broccoli Florets
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The drying of the broccoli was conducted by placing the small florets (250 g) in a thermoshelf of the vacuum dryer (Memmert, model VO 400, Schwabach, Germany). The oven is connected to a vacuum pump (Büchi, model V-100, Flawil, Switzerland), where vacuum inlet is controlled via solenoid valves. The pressure inside the system was set at 10 kPa. The broccoli florets were dried at five temperature levels of 50, 60, 70, 80 and 90 °C. The mass of the samples at a time t (Mt) was recorded using a digital electronic balance (Ohaus SP402; precision ± 0.01 g) every 30 min throughout each drying experiment and stopped when there were no noticeable changes in mass of the samples for the last two data points (equilibrium moisture content). Experiments were conducted in triplicate.
3
Ethanol Extraction of Hemp Seed
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Ethanol (70%) was added to hemp seed in a ratio of 1:10 (w/v) and extracted three times at 25 °C for 24 h. Then, the extract was passed through a filter paper (6.5 cm discs of Whatman No. 1) and evaporated at 35 °C using a rotary vacuum evaporator (V-100; Büchi, Labortechnik, Germany). The ethanol extract was lyophilized and stored at −20 °C for further experiments.
4
Preparation of Lynestrenol-Loaded Transferosomes
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The thin-film hydration method was used for the preparation of the vesicles. The lipid Phospholipon ® 90G and edge activator Tween 80 were employed for the formulation of the vesicles. For the formulation of vesicles, soya phosphatidylcholine, cholesterol, and Tween 80 at different concentrations were selected as the lipid, membrane stabilizer, and edge activator, respectively. The lipid, edge activator, and drug (lynestrenol) were weighed and then dissolved in dichloromethane. This solution was evaporated on a rotary evaporator (Buchi R-100, Switzerland) at 40°C with a vacuum evaporator on 850 mbar (Buchi V-100, Switzerland) until became thin-layer film. Nitrogen gas was allowed to flow through the thin film, and it was then stored in the refrigerator for 24 h. The thin film was then hydrated with pH 7.4 phosphate-buffered saline (PBS) [8, 9] . The formulas of the different transferosomes are shown in Table 1.
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