Day 3 primary T cells and day 1 primary B cells were incubated with peptide and anti-CD40 as described above in 1:1 and 1:10 T/B cell ratios at a total density of 5 × 106 cells per/mL. Three days later they were stained with anti CD4 allophycocyanin (ebioscience clone GK1.5), ICOS PE (ebioscience clone 7E.17G9) and PD1 FITC (ebioscience clone J43) for T-cell marker analysis. CXCR5 bio (BD clone 2G8) was used in combination with streptavidin PerCP (BD). For B-cell staining, GL7 FITC (BD), CD95 PE (BD) and CD19 allophycocyanin (BD, clone 1D3) were used.
Anti cd4 allophycocyanin
Manufactured by Thermo Fisher Scientific
Anti CD4 allophycocyanin is a fluorescent-conjugated antibody that binds to the CD4 receptor on the surface of T helper cells. It is used in flow cytometry applications to identify and quantify CD4+ T cells in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Streptavidin percp, by BD (1 mentions)
Gl7 fitc, by BD (1 mentions)
Cd19 allophycocyanin, by BD (1 mentions)
Pd 1 fitc, by Thermo Fisher Scientific (1 mentions)
Cd95 pe, by BD (1 mentions)
Icos pe, by Thermo Fisher Scientific (1 mentions)
Facscanto 2, by BD (1 mentions)
Anti f4 80 pe, by Thermo Fisher Scientific (1 mentions)
Anti cd11c pe, by BD (1 mentions)
Anti cd11b pe, by BD (1 mentions)
Anti b220 pe, by BD (1 mentions)
Anti ly6c fitc, by BD (1 mentions)
Anti ter119 pe, by Thermo Fisher Scientific (1 mentions)
Anti nk1.1 pe, by BD (1 mentions)
2 protocols using anti cd4 allophycocyanin
1
T-B Cell Activation and Phenotyping
2
Splenic Immune Cell Profiling by FACS
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Single-cell suspensions were prepared from the spleen samples and stained for FACS (fluorescence activated cell sorting) analysis as previously described (Shinno-Hashimoto et al., 2022 (link); Zhang et al., 2021b (link)). The following antibodies were used for immunofluorescence staining: anti-CD3-FITC (x40, cat# 100305: BioLegnd, San Diego, CA), anti-CD4-allophycocyanin (x100, cat# 17-0042-82: eBioscience, San Diego, CA), anti-CD8a-allophycocyanin (x100, cat# 553035: BD Bioscience), anti-NK1.1-PE (x100, cat# 553165: BD Bioscience), anti-Ly6c-FITC (x100, cat# 553104: BD Bioscience), anti-CD11b-PE (x400 diluted using FACS buffer, cat# 553312: BD Bioscience, Franklin Lakes, NJ), anti-CD11c-PE (x100, cat# 557401: BD Bioscience), anti-Ter119-PE (x40, cat# 12-5921-83: eBioscience, San Diego, CA), anti-F4/80-PE (x40, cat# 12-4801-80: Invitrogen), and anti-B220-PE (x200, cat# 553309: BD Bioscience). The stained cells were analyzed using a FACSCanto II and FlowJo software (BD Bioscience).
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