Flow cytometry data were acquired on a FACSCanto (BD Biosciences, San Diego, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). To determine expression of cell surface proteins, mAb were incubated at 4°C for 20–30 min, and cells were fixed using BD Cytofix/Cytoperm solution (BD Biosciences). The following anti-mouse mAb clones were used: CD3 (17A2; BioLegend), CD11a (M17/4; BioLegend), Ly-6C (AL-21; BD Biosciences), KLRG1 (2F1; BioLegend), CD127 (SB/199; eBioscience), CD8a (53-0.67; BioLegend), Thy1.1 (HIS51; eBioscience), CD11b (M1/70; eBioscience), F4/80 (BM8; eBioscience), Ly-6G/C (RB6-8C5; BioLegend). The following anti-human mAb clones were used: CD14 (61D3; Tonbo Biosciences) and CD11b (ICRF44; Tonbo Biosciences). ROS-producing cells were determined via a CellROX Deep Red Kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly, cells were labeled with CellROX Deep Red dye for 30 min at 37°C in complete media prior to Ab labeling.
Cd127 sb 199
Manufactured by Thermo Fisher Scientific
The CD127 (SB/199) is a laboratory equipment product. It is used for the detection and analysis of CD127, a cell surface receptor. The core function of this product is to enable the identification and characterization of CD127-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b m1 70, by Thermo Fisher Scientific (2 mentions)
F4 80 bm8, by Thermo Fisher Scientific (2 mentions)
Cd11a m17 4, by BioLegend (2 mentions)
Thy1.1 his51, by Thermo Fisher Scientific (2 mentions)
Ly6c al 21, by BD (2 mentions)
Ly 6g c rb6 8c5, by BioLegend (2 mentions)
Facscanto, by BD (2 mentions)
Cellrox deep red kit, by Thermo Fisher Scientific (2 mentions)
Cytofix cytoperm solution, by BD (2 mentions)
Cd3 17a2, by BioLegend (2 mentions)
Cd8a 53 0.67, by BioLegend (2 mentions)
Cd14 61d3, by Cytek Biosciences (2 mentions)
Cd11b icrf44, by Cytek Biosciences (2 mentions)
Klrg1 2f1, by BioLegend (2 mentions)
2 protocols using cd127 sb 199
1
Multiparameter Flow Cytometry Analysis
2
Multiparameter Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Flow cytometry data were acquired on a FACSCanto (BD Biosciences, San Diego, CA) and analyzed with FlowJo software (Tree Star, Ashland, OR). To determine expression of cell surface proteins, mAb were incubated at 4°C for 20–30 min, and cells were fixed using BD Cytofix/Cytoperm solution (BD Biosciences). The following anti-mouse mAb clones were used: CD3 (17A2; BioLegend), CD11a (M17/4; BioLegend), Ly-6C (AL-21; BD Biosciences), KLRG1 (2F1; BioLegend), CD127 (SB/199; eBioscience), CD8a (53-0.67; BioLegend), Thy1.1 (HIS51; eBioscience), CD11b (M1/70; eBioscience), F4/80 (BM8; eBioscience), Ly-6G/C (RB6-8C5; BioLegend). The following anti-human mAb clones were used: CD14 (61D3; Tonbo Biosciences) and CD11b (ICRF44; Tonbo Biosciences). ROS-producing cells were determined via a CellROX Deep Red Kit according to the manufacturer’s instructions (Thermo Fisher Scientific). Briefly, cells were labeled with CellROX Deep Red dye for 30 min at 37°C in complete media prior to Ab labeling.
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