Plantinum sybr green supermix udg kit

Total RNA of cultured podocytes under different conditions was extracted using Trizol RNA isolation system (Invitrogen, CA, USA) and reverse transcribed into cDNAs using the Prime ScriptTM RT regent Kit following the instructions provided by the manufacturer (Takara Biotechnology, Dalian, China), and then cDNAs were used for real-time PCR analysis by a Plantinum SYBR Green SuperMix-UDG kit (Takara Biotechnology, Dalian, China). Each reaction was amplified in triplicate. The 2^-ΔΔCt method was used to quantify the relative expression levels of messenger RNA (mRNA). The primers used for qPCR are listed in Supplementary Table S2.

As described previously,^{2 (link)} RNA samples were prepared using Trizol RNA isolation system (Invitrogen) and reverse transcribed into cDNAs using the PrimeScriptTM RT regent Kit following the instructions provided by the manufacturer (Takara Bio Inc, Otsu, Shiga, Japan), and then cDNAs were used for real-time PCR analysis by a Plantinum SYBR Green SuperMix-UDG kit (Takara Bio Inc). The primers used are listed as follows: Cdc42, forward 5′-ATTATGACAGACTACGACCGCT-3′, reverse 5′-AGTGGTGAGTTATCTCAGGCA-3′ Nwasp, forward 5′-ATGCTCCAAATGGTCCCAATC-3′, reverse 5′-CTTGGTGTTCCAATATCTGCCT-3′ YAP, forward 5′-TGAGATCCCTGATGATGTACCAC-3′, reverse 5′-TGTTGTTGTCTGATCGTTGTGAT-3′ Bax, forward 5′-CTGGACCATAGGTCGGAGTG-3′, reverse 5′-AATTCGCCGGAGACACTCG-3′ Bcl-2, forward 5′-GTCGCTACCGTCGTGACTTC-3′, reverse 5′-CAGACATGCACCTACCCAGC-3′ GAPDH, forward 5′-AGGTCGGTGTGAACGG ATTTG-3′, reverse 5′-TGTAGACCATGTAGTTGAGGTCA-3′.

As described previously [6 (link)], RNA samples were prepared using Trizol RNA isolation system (Invitrogen, Carlsbad, CA) and reverse transcribed into cDNAs using the PrimeScriptTM RT regent Kit following the instructions provided by the manufacturer ((Takara Bio Inc, Japan), and then cDNAs were used for real-time PCR analysis by a Plantinum SYBR Green SuperMix-UDG kit (Takara Bio Inc, Japan). The primers used are listed as follows: RhoA, forward 5′-AGCTT GTGGTAAGACATGCTTG-3′, reverse 5′-GTGTCCCATAAAGCCAACTCTAC-3′, Bax, forward 5′-CTGGACCATAGGTCGGAGTG-3′, reverse 5′-AATTCGCCGGAGACACTCG-3′, GAPDH, forward 5′-AGGTCGGTGTGAACGGATTTG-3′, reverse 5′-TGTAGACCATGTAGTTGAGGT CA-3′.