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Recombinant human neuregulin β 1 nrg1

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Recombinant Human Neuregulin β−1 (NRG1) is a protein product used in laboratory research. It is a member of the neuregulin family of growth and differentiation factors. NRG1 plays a role in the development and function of the nervous system.

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Lab products found in correlation

Mesothelin, by Cell Signaling Technology (2 mentions) P erbb2, by Cell Signaling Technology (2 mentions) Igf 1rβ, by Cell Signaling Technology (2 mentions) Tgf β, by Cell Signaling Technology (2 mentions) P jak1 tyr1034 1035, by Cell Signaling Technology (2 mentions) Furin, by Santa Cruz Biotechnology (2 mentions) Jak2 3230s, by Cell Signaling Technology (2 mentions) P jak2 tyr1008, by Cell Signaling Technology (2 mentions) P tyk2 tyr1054 1055, by Cell Signaling Technology (2 mentions) P stat3, by Cell Signaling Technology (2 mentions) P erbb3, by Cell Signaling Technology (2 mentions) Gapdh, by Cell Signaling Technology (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions) Dmem media, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Merck Group (2 mentions) Skov3, by American Type Culture Collection (2 mentions)

2 protocols using recombinant human neuregulin β 1 nrg1

1

Characterization of Ovarian Cancer Cell Lines

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Ovarian cancer cell line OVCAR5 cells were purchased from the National Cancer Institute’s cell line repository. SKOV3, cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and HeyA8 cells were received from the characterized cell line core at MD Anderson Cancer Center. Cells were cultured in DMEM media (GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). ID8 cells (a kind gift from Dr. Weiguo Cui) were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% FBS, 1% penicillin/streptomycin, 1% insulin (5 g/mL), 5 g/mL transferrin, 5 ng/mL sodium selenite (1X ITS; Sigma Cat. No. I3146). We obtained, ERBB2 (#2165), p-ERBB2 (Tyr1248)(#2247), ERBB3 (#12708), p-ERBB3 (Tyr1289), IGF1R-β (#3027), GAPDH (#5174), mesothelin (#99966S), TGF-β (#3711), STAT3 (#9139), p-STAT3 (Tyr705)(#9145), p-STAT3 (Ser727)(#34911), JAK1 (#3344S), p-JAK1(Tyr1034/1035) (#74129S), JAK2 (3230S), p-JAK2 (Tyr1008) (#8082S) TYK2 (#14193S) and p-TYK2 (Tyr1054/1055) (#68790S) from Cell Signaling Technology (Danvers, MA), Furin (#sc-133142) was obtained from Santa Cruz Biotechnology. Recombinant Human Neuregulin β−1 (NRG1), was purchased from PeproTech, Cat# 100–03.
Chen C., Gupta P., Parashar D., Nair G.G., George J., Geethadevi A., Wang W., Tsaih S.W., Bradley W., Ramchandran R., Rader J.S., Chaluvally-Raghavan P, & Pradeep S. (2020). ERBB3-induced furin promotes the progression and metastasis of ovarian cancer via the IGF1R/STAT3 signaling axis. Oncogene, 39(14), 2921-2933.
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2

Characterization of Ovarian Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ovarian cancer cell line OVCAR5 cells were purchased from the National Cancer Institute’s cell line repository. SKOV3, cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and HeyA8 cells were received from the characterized cell line core at MD Anderson Cancer Center. Cells were cultured in DMEM media (GIBCO, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS, Invitrogen Life Technologies, Carlsbad, CA) and 1% penicillin/streptomycin (GIBCO). ID8 cells (a kind gift from Dr. Weiguo Cui) were cultured in Dulbecco’s modified Eagle’s medium (GIBCO) supplemented with 10% FBS, 1% penicillin/streptomycin, 1% insulin (5 g/mL), 5 g/mL transferrin, 5 ng/mL sodium selenite (1X ITS; Sigma Cat. No. I3146). We obtained, ERBB2 (#2165), p-ERBB2 (Tyr1248)(#2247), ERBB3 (#12708), p-ERBB3 (Tyr1289), IGF1R-β (#3027), GAPDH (#5174), mesothelin (#99966S), TGF-β (#3711), STAT3 (#9139), p-STAT3 (Tyr705)(#9145), p-STAT3 (Ser727)(#34911), JAK1 (#3344S), p-JAK1(Tyr1034/1035) (#74129S), JAK2 (3230S), p-JAK2 (Tyr1008) (#8082S) TYK2 (#14193S) and p-TYK2 (Tyr1054/1055) (#68790S) from Cell Signaling Technology (Danvers, MA), Furin (#sc-133142) was obtained from Santa Cruz Biotechnology. Recombinant Human Neuregulin β−1 (NRG1), was purchased from PeproTech, Cat# 100–03.
Chen C., Gupta P., Parashar D., Nair G.G., George J., Geethadevi A., Wang W., Tsaih S.W., Bradley W., Ramchandran R., Rader J.S., Chaluvally-Raghavan P, & Pradeep S. (2020). ERBB3-induced furin promotes the progression and metastasis of ovarian cancer via the IGF1R/STAT3 signaling axis. Oncogene, 39(14), 2921-2933.
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