Nmri nu mice

All experiments were done in accordance with the regulations of animal protection and approved by the Regierungspraesidium Karlsruhe, Germany. Five to six-week-old female NMRI/nu mice (Janvier Laboratory, Le Genest-Saint-Isle, France) received subcutaneous injections into the right flank with 4 × 10⁶ NCH93 cells in 100 µL medium and 100 µL Matrigel (Corning, San Diego, CA, USA). Tumor volume was measured daily by using a digital caliper and it was calculated using the following equation: Volume = (Length × Width²)/2 [38 (link)], where length represents the longest tumor diameter and width represents the perpendicular tumor diameter. When the tumor volume reached 200 mm³, mice were randomly divided into DMSO and ponatinib treatment groups. The dosage of 10 mg per kg bodyweight of ponatinib was administered intraperitoneally daily. The weight of the mice was measured on a daily basis. On day 21, mice were sacrificed, and blood was drawn and immediately sent to the central laboratory of University Hospital Heidelberg for further analysis. Excised tumors were weighed and photographed. Tumor tissues were snap-frozen and stored at −80 °C until further processing.

8–10-wk-old female C57BL/6 and NMRI-nu mice were purchased from Janvier Laboratories. Few experiments were performed on male mice. Xcr1^Cre-mTFP1 and Rosa^26LSL−DTA (B6.129P2-Gt(ROSA)26Sor^{tm1(DTA)Lky/J}) were previously described (Voehringer et al, 2008 (link); Wohn et al, 2020 (link)). Xcr1^Cre-mTFP1 mice were crossed to Rosa^26lsl−DTA mice in which Cre-mediated excision of a loxP-flanked transcriptional STOP element triggers the expression of diphtheria toxin fragment A (DTA), and results in the constitutive ablation of cDC1 in Xcr1^DTA. Mice were housed under a standard 12-h:12-h light–dark cycle with ad libitum access to food and non-acid water, 22°C ± 1°C, and 45–60% humidity, and were maintained under specific pathogen-free conditions at the animal facility of the Centre d’Immunologie de Marseille-Luminy (F1305510). Experimentations were authorized by the Ethical Committee for Animal Experimentation (#30566–2021032215496999 v2; APAFIS). Collaborative experiments using Xcr1^DTA mice (Wohn et al, 2020 (link)) were performed in B. Malissen’s laboratory (#26488–2020070612584424 v2; APAFIS).