The cytotoxicity and chikungunya virus (Togaviridae, Alphavirus, SFV complex) strain S-27 [ATCC® VR-64™, obtained from American Type Culture Collection (ATCC), Manassas, VA] antiviral assays were performed utilizing the neutral red assay, and the virus yield reduction assay, in Vero 76 cells. The cells were maintained in MEM (MEM/EBSS, Hyclone, Logan, UT) supplemented with 5% fetal bovine serum (FBS). NC was dissolved in dimethyl sulfoxide (DMSO) at 20 mM, and then further diluted in the test medium half log dilutions to a final DMSO concentration of ≤ 5% DMSO with 1,000, 320, 100, 32, 10, 3.2, 1.0, and 0.32 μM of the compound for antiviral testing. The utilized positive control was interferon alfacon-1 (INFERGEN™), a consensus interferon-α 1. The assays were conducted in triplicate and treated statistically.
Atcc vr 64
Manufactured by American Type Culture Collection
ATCC® VR-64™ is a laboratory product offered by American Type Culture Collection. It is a specific viral strain that can be used for various research and testing purposes. No further details about its core function or intended use are provided to maintain an unbiased and factual approach.
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2 protocols using atcc vr 64
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Chikungunya Virus Antiviral Assays in Vero 76 Cells
2
Chikungunya Virus Antiviral Assays in Vero Cells
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The cytotoxicity and chikungunya virus (Togaviridae, Alphavirus, SFV complex) strain S-27 [ATCC® VR-64™, obtained from American Type Culture Collection (ATCC), Manassas, VA] antiviral assays were performed utilizing the neutral red assay, and the virus yield reduction assay, in Vero 76 cells. The cells were maintained in MEM (MEM/EBSS, Hyclone, Logan, UT) supplemented with 5% fetal bovine serum (FBS). NC was dissolved in dimethyl sulfoxide (DMSO) at 20 mM and then further diluted in the test medium half log dilutions to a final DMSO concentration of ≤ 5% DMSO with 1000, 320, 100, 32, 10, 3.2, 1.0, and 0.32 μM of the compound for antiviral testing. The utilized positive control was interferon alfacon-1 (INFERGEN™), a consensus interferon-α 1. The assays were conducted in triplicate and treated statistically.
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