Anti-human NKp46 antibodies (clone 195,314, R&D Systems, Minneapolis, MN, USA) and anti-human CD16 antibodies (clone 3G8, Thermo Fisher Scientific, Waltham, MA, USA) (both 5 μg/mL) were prepared in phosphate-buffered saline (PBS; Kohjin Bio, Saitama, Japan) containing 0.1% human serum albumin (FUJIFILM Wako Pure Chemical, Tokyo, Japan). A 1.5 or 0.7 mL antibody solution was transferred to 24- or 12 well-well plates (Corning, Steuben, NY, USA) and incubated at 4 °C for >12 h for antibody immobilization. The antibody solution was then removed from the flask, the flask was washed with PBS, and subsequently used for human NKC culturing.
Anti human nkp46 antibodies clone 195 314
Manufactured by R&D Systems
Sourced in United States
Anti-human NKp46 antibodies (clone 195,314) are specific for the NKp46 receptor expressed on natural killer (NK) cells. NKp46 is a key activating receptor involved in the cytolytic function of NK cells.
Automatically generated - may contain errors
2 protocols using anti human nkp46 antibodies clone 195 314
1
Immobilization of NKp46 and CD16 Antibodies
2
Immobilization of Anti-NKp46 and Anti-CD16 Antibodies
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Anti-human NKp46 antibodies (clone 195, 314, R&D Systems, Minneapolis, MN, USA) and anti-human CD16 antibodies (clone 3G8, Thermo Fisher Scientific, Waltham, MA, USA) (both, 5 mg/mL) were prepared in phosphate-buffered saline (PBS; Kohjin Bio, Saitama, Japan) containing 0.1% human serum albumin (FUJIFILM Wako Pure Chemical, Tokyo, Japan). The antibody solution (1.5 or 0.7 mL) was transferred to 24- or 12-well plates (Corning, Steuben, NY, USA) and incubated at 4 °C for >12 h for antibody immobilization. The antibody solution was then removed from the flask, the flask was washed with PBS, and subsequently used for human NKC culturing.
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