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3 protocols using nh3 h2o

1

Synthesis of Spherical MnCO3 Precursors

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LiOH·H2O (99.0%, AR) and ethanol (99.7%, AR) were purchased from Aladdin. MnSO4·H2O (99.0%, AR), NH4HCO3 (21.0%, AR), NH3·H2O (25%, AR), and H2SO4 (98.0 wt.%) were supplied by XiLong Chemical Co. Ltd. All reagents were used without further purification. The spherical MnCO3 was first prepared by a general chemical precipitation method. In a typical synthesis, 0.3042 g MnSO4·H2O and 1.4231 g NH4HCO3 were dissolved in 45 mL deionized water and 5 mL ethanol, respectively, to form a transparent solution. After the complete dispersion of the MnSO4 and NH4HCO3 solutions, the NH4HCO3 solution was added to the MnSO4 solution rapidly with vigorous stirring. A certain amount of NH3·H2O (10.0% v/v.) or H2SO4 (10.0% v/v.) was then added dropwise to adjust the pH value of the suspension to 7.5. The milky white suspension was stirred for 3 h at room temperature and maintained for 5 h. The powder was obtained by filtrating, washing, and drying in the air at 80°C for 24 h to obtain spherical MnCO3 precursors.
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2

Porcine Liver and Cell Line Protocol

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Fresh porcine liver was purchased from the supermarket. L929 cell line and 4T1 cell line were donated by Zhengzhou Institute of Emerging Industrial Technology (Zhengzhou, China) and Cancer Hospital of China Medical University (Shenyang, China), respectively. NaCl, KCl, KH2PO4, and Na2HPO4·12H2O were provided by Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Triton X-100, sodium dodecyl sulfate (SDS), and trypsin were provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). NH3·H2O was provided by Xilong Scientific Co., Ltd. (Shantou, China). EDTA, glutaraldehyde, and ethyl alcohol were provided by Damao Chemical Reagent Factory (Tianjin, China). Hematoxylin and eosin (H&E) dye, Masson dye, and pepsin were provided by Beijing Coolaber Science & Technology Co., Ltd. (Beijing, China). Hoechst 33324, Calcein-AM, and propidium iodide (PI) were provided by Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). α-DMEM and penicillin-streptomycin were provided by Sigma-Aldrich Inc. (St. Louis, MO, USA). RPMI 1640 was provided by Procell Life Science & Technology Co., Ltd. (Wuhan, China). CCK-8 kit was provided by Dojindo Laboratories (Kumamoto, Japan).
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3

Preparation of Catalytic Nanoparticles

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Al(NO3)3·9H2O (99.99%) and NH3·H2O (25~28%) were purchased from Xilong chemical Co., Ltd. (Shantou, China). (NH4)2HPO4 (99.99%) was obtained from Tianjin Fengchuan chemical reagent technology Co., Ltd. (Tianjin, China). Ce(NO3)3·6H2O (99.99%) and Zr(NO3)4·5H2O (99.99%) were purchased from West Asia chemical technology (Shandong) Co., Ltd. (Linyi, China) Pd(NO3)2 (24% Pd content) was obtained from Sino-Platinum Metals Co., Ltd. (Kunming, China). All chemicals were used as received without purification.
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