IBs were suspended in 50 mM Tris-HCl buffer, pH 8.0, containing SDS from 0.01 to 0.1% or urea from 1 to 8 M, and incubated for a specified time (from 5 min to 24 h). Turbidity of the samples was measured at 350 nm with a μQuant spectrophotometer (BioTEK Instruments, Inc., USA). Fluorescence intensity of the PldA-GFP IBs solutions in urea and SDS was recorded with a FL-600 Fluorescence/Absorbance Plate Reader (BioTEK Instruments, USA) with excitation wavelength at 495 nm and emission at 530 nm. The experiments were performed in three biological replicates. Results show the mean ± standard deviation (SD).
Fl 600 fluorescence absorbance plate reader
Manufactured by Agilent Technologies
Sourced in United States
The FL-600 Fluorescence/Absorbance Plate Reader is a versatile instrument designed for fluorescence and absorbance measurements. It can perform various types of assays, including cell-based, biochemical, and molecular biology assays, in a high-throughput microplate format.
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Lab products found in correlation
2 protocols using fl 600 fluorescence absorbance plate reader
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Optimization of Inclusion Body Solubility
2
Caspase3 Activity Assay Protocol
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