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M125 microscope

Manufactured by Jenoptik

The M125 microscope is a high-quality optical instrument designed for a variety of laboratory applications. It features a magnification range of 8x to 125x, providing users with the ability to observe samples in detail. The microscope is equipped with a built-in LED illumination system and offers a wide field of view for comfortable observation.

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2 protocols using m125 microscope

1

Imaging Malpighian Tubules in Flies

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Flies were quickly frozen in −80°C and imaged using a Leica M125 microscope with a ProgRes C14 plus camera (Jenoptik) and the ProgRes CapturePro 2.8 Jenoptik Optical system. The Malpighian tubules from female and male adult flies were dissected and mounted in 50% glycerol before imaging. Images were handled using ImageJ, Fiji edition (Schindelin et al., 2012 (link)).
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2

Examining CG18549 Knockdown Effects

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F1 progenies from da-GAL4 > w1118 (Driver ctrl), w1118 > CG18549 RNAi (RNAi ctrl) and da-GAL4 > CG18549 RNAi (CG18549 knockdown) were collected and examined. Both CG18549 RNAi lines were used, and the counting was performed on progenies from three crosses of each genotype. The mean (±95% CI) are plotted for male and female progenies separated as well as merged for each RNA line. Differences were analyzed using Kruskal–Wallis with Dunn’s comparison. Progenies were investigated for developmental flaws using a Leica M125 microscope with a ProgRes C14 plus camera (Jenoptik) and the ProgRes CapturePro 2.8 Jenoptik Optical system.
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