Immunohistochemistry was performed as previously described. In brief, after deparaffinizing the sections were treated using 0.3% H2O2 at room temperature for 10 min to quench endogenous peroxidase. Next, 1% BSA was used to incubate sections for 30 min at 37°C to block non-specific staining. Then, sections were incubated with primary antibody overnight at 4°C. After rinsing with PBS, sections were incubated with anti-Ecad 1:200 (Proteintech, China) or anti-SMA 1:200 (Proteintech, china) antibody at 37°C for 30 min and HRP-conjugated streptavidin at 37°C for 30 min, in turn. Finally, sections were incubated with 3.3′‐diaminobenzidine tetrahydrochloride (DAB) to show positive staining. Sections were observed under a high magnification (400×) with a microscope (Olympus, Japan).
Anti sma
Manufactured by Proteintech
Sourced in China
Anti-SMA is a lab equipment product manufactured by Proteintech. It is used for the detection and visualization of smooth muscle actin (SMA) in biological samples.
Automatically generated - may contain errors
2 protocols using anti sma
1
Immunohistochemical Analysis of Cell Markers
2
Immunofluorescence Analysis of Adenomyosis Tissue
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The paraffin-embedded adenomyosis tissue sections were baked in the oven at 65 °C for 12 h. After deparaffinization and blocking, the antigen–antibody reaction was incubated overnight at 4 °C. Immunofluorescence staining with following antibodies: anti-Vimentin (Cat No: 10366–1-AP, Proteintech), anti-E-cadherin (Cat No: 610181, BD, Biosciences), anti-VEGF (Cat No: 19003–1-AP, Proteintech), anti-SMA (Cat No: 55135–1-AP, Proteintech). Fluorescence was visualized with Olympus X71.
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