Ab216262

Manufactured by Abcam

Ab216262 is a laboratory equipment product. It is a tool designed for use in scientific research and experimentation.

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Lab products found in correlation

Ab192256, by Abcam (1 mentions) Ab49945, by Abcam (1 mentions) Mef2c, by Cell Signaling Technology (1 mentions) Slcj8103, by Merck Group (1 mentions) Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Hif 1α, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Sc 52746, by Santa Cruz Biotechnology (1 mentions) Sc 126, by Santa Cruz Biotechnology (1 mentions) Sc 7480, by Santa Cruz Biotechnology (1 mentions) Sc 7341, by Santa Cruz Biotechnology (1 mentions) Chemidoc imaging system, by Bio-Rad (1 mentions) Image laboratory software version 5, by Bio-Rad (1 mentions) Sc 56053, by Santa Cruz Biotechnology (1 mentions) Sc 7382, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ab216262

Immunofluorescence Analysis of Spinal Chondrocytes

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Spine tissues of P7 CKO and WT newborn mice were collected, and 6-μm frozen sections were prepared. The sections were permeabilized with 0.1% Triton X-100 for 5 min and blocked in 5% BSA for 30 min at room temperature. Then, sections were incubated with primary antibodies against mouse MAPK7 (Cell Signaling Technology, 3372s; 1:200), COL10A1 (Abcam, ab49945; 1:500), RUNX2 (Abcam, ab192256; 1:400), MMP13 (Abcam, ab39012; 1:400), PTEN (Cell Signaling Technology, 9559s; 1:400), p-AKT (Cell Signaling Technology, 9018s; 1:200), MEF2C (Cell Signaling Technology, 5030s; 1:200), HIF1α (Cell Signaling Technology, 36169s; 1:200), and Cre recombinase (Abcam, ab216262; 1:500) overnight at 4 °C. The following incubation with the secondary antibody Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen, S11227; 1:1000) was performed at room temperature for 1 h. Nuclei were labeled with DAPI (Sigma, SLCJ8103) for 5 min. The immunofluorescence staining images were captured with fluorescence microscopy (Olympus, BX63, Japan) and quantified using Image J software.

Wu C., Liu H., Zhong D., Yang X., Liao Z., Chen Y., Zhang S., Su D., Zhang B., Li C., Tian L., Xu C, & Su P. (2023). Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes & Diseases, 11(2), 964-977.

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Protein Analysis in Mouse Tissues

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For protein analysis, protein samples (3 mice/group) were harvested from the three tissue regions collected for protein assay and immunoblotting. Brain tissue samples were homogenized in a radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors, followed by bicinchoninic acid assay. Equal amounts of proteins extracts were run on 10% SDS-PAGE, followed by immunoblotting analysis. Antibodies for YY1, Cre recombinase, p53, Bax, Bcl-2, caspase-3, CXCL10, TNF-α, and β-actin at 1:1000-1:2000 dilution were used, followed by horseradish peroxidase-conjugated secondary antibody (1:5000 dilution). Antibodies for YY1 (sc-7341), p53 (sc-126), caspase-3 (sc-56053), CXCL10 (sc-374092), TNF-α (sc-52746), Bax (sc-7480), Bcl-2 (sc-7382), and β-actin (sc-47778) were obtained from Santa Cruz Biotechnology and antibody for Cre recombinase (ab216262) were from Abcam. All blots were developed using a West Pico PLUS chemiluminescence substrate detection kit (Pierce, Rockford, IL), followed by blot imaging and quantification with the Bio-Rad ChemiDoc Imaging System and Image Laboratory Software version 5.2.1 (Bio-Rad), respectively.

Pajarillo E., Nyarko-Danquah I., Digman A., Vied C., Son D.S., Lee J., Aschner M, & Lee E. (2022). Astrocytic yin yang 1 (YY1) is critical for murine brain development and protection against apoptosis, oxidative stress, and inflammation. Glia, 71(2), 450-466.

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