All the extracts and their fractions with cytotoxic potential were analyzed by HPLC-PDA (high-performance liquid Chromatography coupled with a photo diode array detector) (Shimadzu Chromatograph equipped with two Shimadzu LC-10AD pumps, Shimadzu SIL 10A auto-injector, UV-Vis array detector model Shimadzu SPD/MX/AVP; the data acquisition and processing were treated on the Shimadzu CLASSLC10 software version 1.64A) in analytical mode (gradient elution 5% to 100% MeOH; analytical column “Luna” Phenomenex® C18 (250 × 4.6 mm, 4 μm) and UV detector monitored at 254 nm; injection volume: 40 μL, run time: 40 min, flow: 1 mL min−1), in order to rapidly obtain a profiling of the bioactive compounds.
Spd mx avp
Manufactured by Shimadzu
The SPD/MX/AVP is a high-performance centrifuge system designed for a variety of laboratory applications. It features a powerful motor and advanced temperature control capabilities to ensure precise and consistent sample processing. The SPD/MX/AVP is built to withstand heavy-duty use and offers reliable performance for a range of centrifugation requirements.
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2 protocols using spd mx avp
1
HPLC-PDA Analysis of Cytotoxic Extracts
2
Phytopreparations Analysis by RP-HPLC-PDA
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RP-HPLC-PDA analysis of all the phytopreparations was carried out in a Shimadzu Chromatograph equipped with two Shimadzu LC-10AD pumps, Shimadzu SIL 10 A auto-injector, UV-Vis array detector model Shimadzu SPD MX AVP. Briefly, the data acquisition and processing have treated on the Shimadzu Classlc10 software (version 1.64A). For these experimental measured at room temperature, were used a Phenomenex C18-Hydro (250 x 4.6 mm, 4 μm) with a flow of 1 mL min -1 , the injection volume was 30 μL. In addition, the 1 H-NMR (500 MHz) spectra of the samples were then obtained on the Varian Inova 500® Spectrometer. For comparison of micro molecule extraction, all samples were analyzed at the same concentration (dissolving 10 mg of the dried material in 300 μL deuterated water).
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