2, 2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,6-dimethoxyphenol (DMP), guaiacol, toluidine, 3-aminobenzoic acid and phenyldiamine were purchased from Sigma-Aldrich (St. Louis, MO), respectively. All other chemicals and reagents were of analytical grade and were purchased from commercial sources, unless otherwise stated. Reagents for polymerase chain reaction (PCR), Ex-Taq DNA polymerase, the genomic DNA extraction kit, and the pGEM-T easy vector were purchased from Promega (Madison, WI). T4 DNA ligase and restriction enzymes were obtained from New England Biolabs (Beverly, MA). Plasmid pPICZαA and Zeocin were purchased from Invitrogen (Carlsbad, CA).
Ex taq dna polymerase
Manufactured by Promega
Sourced in United States
Ex-Taq DNA polymerase is a thermostable DNA polymerase enzyme used for DNA amplification in polymerase chain reaction (PCR) applications. It possesses 5'-3' DNA polymerase and 5'-3' exonuclease activities.
Automatically generated - may contain errors
Lab products found in correlation
Genomic dna extraction kit, by Promega (3 mentions)
Pgem t easy vector, by Promega (2 mentions)
Plasmid isolation kit, by Qiagen (2 mentions)
Oligonucleotide primers, by Bioneer (2 mentions)
Guaiacol, by Merck Group (1 mentions)
Ppiczαa, by Thermo Fisher Scientific (1 mentions)
T4 dna ligase and restriction enzymes, by New England Biolabs (1 mentions)
3 aminobenzoic acid, by Merck Group (1 mentions)
Zeocin, by Thermo Fisher Scientific (1 mentions)
2 2 azino bis, by Merck Group (1 mentions)
Nickel nitrilotriacetic acid superflow column, by Qiagen (1 mentions)
Pet28a expression vector, by Qiagen (1 mentions)
T4 dna ligase, by New England Biolabs (1 mentions)
Ni nta superflow column, by Qiagen (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
3 protocols using ex taq dna polymerase
1
Enzyme Assay with ABTS and Phenolics
2
Molecular Cloning and Protein Purification
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Ex-Taq DNA polymerase, a genomic DNA extraction kit, pGEM-T easy vector, and reagents for polymerase chain reaction were purchased from Promega (Madison, WI, USA). T4 DNA ligase and restriction enzymes were purchased from New England Biolabs (Ipswich, MA, USA). The pET28a expression vector, plasmid isolation kit, and nickel-nitrilotriacetic acid superflow column for His-tag protein purification were obtained from Qiagen (Hilden, Germany)20 (link). Oligonucleotide primers were obtained from Bioneer (Daejeon, Republic of Korea)29 (link). Electrophoresis reagents were provided by Bio-Rad (Hercules, CA, USA), and all chemicals used in assays were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Nanoparticle-Based Protein Expression Protocol
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The Ex-Taq DNA polymerase, a genomic DNA extraction kit, and PCR reagents were purchased from Promega (Madison, USA). Restriction enzymes were purchased from New England Biolabs (MA, USA)36 (link). The expression vector, plasmid isolation kit, and NiNTA superflow column were obtained from Qiagen (Hilden, Germany)36 (link). Oligonucleotide primers were obtained from Bioneer (Daejeon, South Korea). Chemicals used in assays were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nanoparticles were obtained from Nanostructured and Amorphous Materials (Houston, TX, USA) as described previously21 (link).
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