The serum nitrite assay was determined using a Sigma-Aldrich® Nitrite Assay or Griess Reagent Kit (St. Louis, MO, USA) according to the manufacturer’s protocol. The assay was done in duplicates and previously known positive and negative controls were included simultaneously. A standard curve was prepared using the standard reagents. The standard curve was used to derive the concentrations of nitrite in the test samples with the absorbance read at 540nm and the nitrite concentrations were expressed as μM.
Nitrite assay or griess reagent kit
Manufactured by Merck Group
Sourced in United States
The Nitrite Assay or Griess Reagent Kit is a laboratory tool used for the colorimetric detection and quantification of nitrite (NO2-) in various biological samples. It provides a simple and reliable method to measure nitrite levels, which is an important indicator of nitric oxide (NO) metabolism and signaling.
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Lab products found in correlation
2 protocols using nitrite assay or griess reagent kit
1
Serum Nitrite Quantification Protocol
2
Measuring Serum Nitrite Levels
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The serum nitrite assay was determined using a Sigma-Aldrich® Nitrite Assay or Griess Reagent Kit (St. Louis, MO, USA) according to the manufacturer’s protocol. The assay was done in duplicates and previously known positive and negative controls were included simultaneously. A standard curve was prepared using the standard reagents. The standard curve was used to derive the concentrations of nitrite in the test samples with the absorbance read at 540 nm and the nitrite concentrations were expressed as μM.
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