Spf c57bl 6 male mice

Germ-free C57BL/6J male mice (18–20 g, 5 weeks old) were purchased from Clea Japan Inc. (Tokyo, Japan) and bred under aseptic conditions according to The Guidelines for Laboratory Germ-free Animals Care and Usage. SPF C57BL/6 male mice (19–21 g, 5 weeks old) were purchased from Koatech (Pyungtaek-shi, Korea). All mice were maintained in plastic cages with the raised wire floor at 20–22 °C and 50 ± 10% humidity, and fed standard laboratory chow and water ad libitum. The mice were acclimatized for one week before usage in the experiments. All animal experiments were approved by The Committee for the Care and Use of Laboratory Animals in Kyung Hee University (Seoul, Korea) and carried out according to The Kyung Hee University Guidelines for Laboratory Animals Care and Usage (IACUC No., KHSASP-18-115 and 19-290).

SPF C57BL/6 male mice (19–22 g, 6 weeks old) were purchased from Koatech (Pyungtaek-shi, Korea). All mice were maintained in plastic cages with the raised wire floor at 20–22 °C and 50 ± 10% humidity, and fed standard laboratory chow and water ad libitum. Each group consisted of 8 mice (4 mice in each cage). The animals were acclimatized for one week before usage in the experiments. All animal experiments were approved by The Committee for the Care and Use of Laboratory Animals in Kyung Hee University (Seoul, Korea) and performed in the accordance to The Kyung Hee University Guidelines for Laboratory Animals Care and Usage (IACUC No., KHSASP-20018). This study additionally adheres to standards articulated in the ARRIVE guidelines.