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12 well tissue culture treated plates

Manufactured by Corning
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The 12-well tissue culture-treated plates are a laboratory equipment product designed for cell and tissue culture applications. These plates provide a standardized and controlled environment for the cultivation and growth of various cell lines. The plates feature 12 individual wells with a treated surface to promote cell attachment and proliferation. This product is intended to support fundamental research and experimentation in the life sciences.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Silencer select, by Thermo Fisher Scientific (1 mentions) Viromer green, by BioNTech (1 mentions) Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions) X tremegene sirna transfection reagent, by Roche (1 mentions) Sirna duplexes, by Horizon Discovery (1 mentions)

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12-well plates (590 citations) 12-well culture plates (86 citations) Tissue culture plates (66 citations) 12-well tissue culture plates (61 citations) Tissue culture treated plates (22 citations)

3 protocols using 12 well tissue culture treated plates

1

Knockdown of CD26 and CASP1 in Cell Lines

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For CD26 KD analysis, H2452 cells were grown in 12‐well tissue culture‐treated plates (Corning, New York, NY) and transfected with either 20 pmol/L of small interfering RNA (siRNA) targeted CD26 (Silencer® Select, s4254 as #1 and s4255 as #2, Ambion, Austin, TX), or control siRNA (Silencer® Select, Negative Control #1) using Lipofectamine RNAiMAX (Invitrogen). For CASP1 KD analysis, THP‐1 cells were grown in 12‐well tissue culture‐treated plates (Corning) and transfected with either 100 pmol/L of small interfering RNA (siRNA) targeted CASP1 (Silencer® Select, s2407) using Viromer® GREEN (Lipocalyx, Weinbergweg, Germany). After 48 h, cells were harvested and used for the individual experiments.
Horio D., Minami T., Kitai H., Ishigaki H., Higashiguchi Y., Kondo N., Hirota S., Kitajima K., Nakajima Y., Koda Y., Fujimoto E., Negi Y., Niki M., Kanemura S., Shibata E., Mikami K., Takahashi R., Yokoi T., Kuribayashi K, & Kijima T. (2020). Tumor‐associated macrophage‐derived inflammatory cytokine enhances malignant potential of malignant pleural mesothelioma. Cancer Science, 111(8), 2895-2906.
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2

Mandible and Tibia-Derived BMSC Proliferation

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Mandible and tibia-derived BMSCs (Passage 1–4) were seeded on 12-well tissue culture-treated plates (Corning, NY) at 5,000 cells per well and resuspended in regular growth media as described above. Cultures were then incubated at 37 °C and 5% CO2 and designated for harvest according to predetermined time points. Cells were then detached using TrypLE dissociation reagent every two days for two weeks and enumerated using a hemacytometer to record the final cell number. Triplicate measurements of each time point were used and two measurements were made of each sample to minimize measurement error. Remaining wells were fed with fresh growth media every two days until the time point at which they were designated for enumeration. The population doubling time for each culture was then calculated based on the longitudinal cell counting values up to 13 days by using an online calculator (http://www.doubling-time.com/compute_more.php) which uses a least squares fitting exponential regression.
Lloyd B., Tee B.C., Headley C., Emam H., Mallery S, & Sun Z. (2017). Similarities and Differences between Porcine Mandibular and Limb Bone Marrow Mesenchymal Stem Cells. Archives of oral biology, 77, 1-11.
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3

siRNA-mediated knockdown of DNA repair genes

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siRNA duplexes targeting Msh2, Msh6 and LacZ were purchased from Dharmacon (Thermo Scientific). Sense sequences of the siRNA are: Msh2, 5′-ACAGAAUAGAGGAGAGAUUUU-3′; Msh6, 5′-GAAUACGAGUUGAAAUCUAdTdT-3′; LacZ, 5′-CGUACGCGGAAUACUUCGAdTdT-3′. HeLa cells were seeded at a density of 0.3 X 105 cells per well in 12-well tissue culture treated plates (Corning) and maintained at 37°C for 24 hours. Transfections were performed with a final siRNA concentration of 100 nM using X-tremeGENE siRNA transfection reagent (Roche) diluted in OptiMEM I Reduced Serum Medium (Invitrogen). DMEM supplemented with 30% FBS was added 4 hours post-transfection to achieve a final FBS concentration of 10% in the wells. After 24 hours, siRNA transfection was repeated for each set. Cells were maintained at 37°C for an additional 48 hours and NEU damage was induced before lysis using same procedure as described earlier.
Bodakuntla S., V L.A., Sural S., Trivedi P, & Lahiri M. (2014). N-nitroso-N-ethylurea activates DNA damage surveillance pathways and induces transformation in mammalian cells. BMC Cancer, 14, 287.
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