The effect of spautin‐1 on intracellular ROS generation was measured using a DCFH‐DA reactive oxygen species ROS fluorescence probe (Solarbio). Cells (3 × 105 per well) were seeded in a 6‐well plate to allow attachment and then exposed to spautin‐1 for 0, 3, 6 or 12 hours. Then, the cells were incubated with DCFH‐DA for 30 minutes according to the manufacturer's instruction. The intracellular ROS generation was measured by a DxP Athena cytometer (Cytek), and the data were analysed by the FlowJo10 software.
Dxp athena cytometer
Manufactured by Cytek
Sourced in United States
The DxP Athena cytometer is a flow cytometry instrument developed by Cytek. It is designed to analyze and sort cells based on their physical and fluorescent properties. The DxP Athena cytometer utilizes a multi-color fluorescence detection system to provide high-resolution data on multiple cellular parameters simultaneously.
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Lab products found in correlation
2 protocols using dxp athena cytometer
1
Quantifying Intracellular ROS with Spautin-1
2
Quantifying Cell Apoptosis with Annexin V-FITC
Check if the same lab product or an alternative is used in the 5 most similar protocols
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According to the manufacturer’s instructions, the Annexin V-FITC Apoptosis Detection Kit (Absin, Shanghai, China) was utilized to evaluate the level of cell apoptosis. Initially, the cells were harvested and washed with cold PBS. Then, they were resuspended in 300 μL of binding buffer at a concentration of 106 cells/ml. The cell suspension was then subjected to a 10-min incubation on ice in the dark with Annexin V-FITC, followed by a 5-min incubation with PI. Finally, the samples were analyzed using a DxP Athena™ Cytometer (Cytek Biosciences, USA), as previously described [42 (link)].
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