Phospholipase c from b cerus

Chloroform and ethanol (HPLC grade) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol was LC-MS grade from Fisher Scientific (Pittsburgh, PA, USA). 2-(4-Morpholino)ethane sulfonic acid (MES) was BioReagent grade also from Fisher Scientific (Pittsburgh PA, USA). Water was purified by a milliQ system (EMD Millipore, Billerica, MA, USA). 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) was purchased from Thermo Scientific/Pierce Biotechnology (Rockford, IL, USA). Phospholipase C from B. cerus, ammonium acetate, and 2,5-dihydroxybenzoic acid (DHB) were also purchased from Sigma-Aldrich (St. Louis, MO, USA).

Water for tissue treatment was purified by a Milli-Q system (EMD Millipore, Billerica, MA). 2-(4-Morpholino)ethanesulfonic acid (MES) was BioReagent grade from Fisher Scientific (Pittsburgh PA). 1-Ethyl-3-[3-(dimethylamino)propyl] carbodiimide hydrochloride (EDC) was purchased from Thermo Scientific/Pierce Biotechnology (Rockford, IL). Phospholipase C from B. cerus and ammonium acetate were purchased from Sigma-Aldrich (St. Louis, MO). Harris Hematoxylin, Eosin Y, and Bluing Reagent for hematoxylin and eosin (H&E) staining were purchased from ThermoFisher Scientific (Waltham, MA). Histology grade ethanol and xylenes were also from ThermoFisher Scientific. Chloroform, water, and ethanol for cleaning the copper substrate were HPLC grade from OmniSolv EMD Millipore (Billerica, MA).