For the IHC study, a total of 80 pairs of HCC cancerous and adjacent normal tissues were obtained from the Affiliated Tumor Hospital of Guangxi Medical University (Nanning, China) in 2014. IHC analysis was performed on paired formalin-fixed and paraffin-embedded cancerous and adjacent normal tissues from HCC patients according to standard procedures. Briefly, paraffin sections were hydrated, followed by microwave antigen retrieval and blocking of endogenous peroxidase activity with 3 % H2O2. Tissue sections were blocked for non-specific binding sites with 10 % goat serum and then incubated with RBP4 primary antibody (1:150, Anti-RBP4 antibody, Abcam, Cambridge, MA, USA) at 4 °C overnight. Thereafter, the sections were incubated with biotinylated secondary antibody (Envision™ Detection kit; Gene Tech, Shanghai, China) for 2 h and stained with diaminobenzidine. Positive immunoreactivity (brown staining) were analyzed semi-quantitatively into 4 ranks (from low to high: -, +, ++, and +++) as described previously [27 (link)].
Anti rbp4 antibody
Manufactured by Abcam
Sourced in United States, United Kingdom
The Anti-RBP4 antibody is a laboratory reagent designed for the detection and analysis of the RBP4 protein. RBP4 is a retinol-binding protein involved in the transport of retinol (vitamin A) in the bloodstream. This antibody can be used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to measure or visualize the presence and distribution of RBP4 in biological samples.
Automatically generated - may contain errors
2 protocols using anti rbp4 antibody
1
Immunohistochemical Analysis of HCC
2
Western Blot Analysis of RBP4
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The liver and adipose tissues were homogenized in lysis buffer (20 mmol/L HEPES, pH 7.5, 150 mmol/L NaCl, 1 mmol/L EDTA, 1% Triton X-100, 0.5 mmol/L Na 3 VO 4 ) with protease inhibitors (Roche Molecular Biochemicals, Mannheim, Germany). Cultured adipocytes and hepatocytes were lysed in the same lysis buffer, and crude homogenates were centrifuged at 12,000 rpm for 15 min at 4 ºC. Protein concentration was measured by BCA protein quantification Kit (Beyotime, Shanghai, China). Equal amount of proteins were separated on 10% SDS-PAGE gels, and then transferred onto nitrocellulose membranes (Millipore, Billerica, MA, USA). Membranes were blocked with 5% non-fat dried milk (NFDM) (Bright dairy, China) in TBST (Tris-buffered saline/Tween-20) for 1 h at room temperature, and then incubated with anti-RBP4 antibody (Abcam, UK) overnight at 4 °C. After three washes with TBST, 10 minutes each, membranes were
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