WE-68 cells were a gift from Dr F. van Valen (Münster, Germany). SK-ES-1 and HeLa cells were purchased from the DSMZ (Braunschweig, Germany). A673 cells were purchased from Sigma Aldrich (Deisenhofen, Germany). WE-68, SK-ES-1 and HeLa cells were cultured in RPMI 1640 medium and A673 cells were cultured in DMEM (Lonza, Cologne, Germany). Media were supplemented with 10% foetal calf serum (Capricorn Scientific, Ebsdorfergrund, Germany), 2 mM l -glutamine, 100 units/ml penicillin G sodium and 100 µg/ml streptomycin sulphate (Lonza). All tissue culture vessels used for the cultivation of ES cells were coated with rat tail collagen (Merck, Darmstadt, Germany) at a concentration of 5 µg/cm2. Cells were maintained at a temperature of 37 °C in a humidified 5% CO2 incubator and routinely passaged at a confluence of ~ 90%. Cells were tested to be negative for mycoplasma with the qPCR Mycoplasma Test Kit from Applichem (Darmstadt, Germany).
A673 cells
Manufactured by Merck Group
Sourced in Germany
A673 cells are a human cell line derived from an Ewing's sarcoma, a type of bone cancer. They are commonly used in cell biology research as a model system for studying cancer mechanisms and testing potential therapeutic agents. The A673 cell line is well-characterized and widely available for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Lonza (1 mentions)
Foetal calf serum, by Capricorn (1 mentions)
Rat tail collagen, by Merck Group (1 mentions)
Sk es 1, by Leibniz Institute DSMZ (1 mentions)
Streptomycin sulphate, by Lonza (1 mentions)
Qpcr mycoplasma test kit, by AppliChem (1 mentions)
L glutamine, by Lonza (1 mentions)
Penicillin g sodium, by Lonza (1 mentions)
Dmem ham s f12, by Harvard Bioscience (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Harvard Bioscience (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Ku55933, by Selleck Chemicals (1 mentions)
Ve 821, by Selleck Chemicals (1 mentions)
Auy922, by Selleck Chemicals (1 mentions)
2 protocols using a673 cells
1
Cell Culture Protocol for Sarcoma Cell Lines
2
Cytotoxic Compound Screening in Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture and treatment Dr. F. van Valen (Münster, Germany) kindly provided WE-68 cells. A673 cells were purchased from Sigma Aldrich. HCT116 p53 wt and p53 -/-colon cancer cells were a gift from Dr. B. Vogelstein (Baltimore, MD, USA). WE-68 cells were maintained in RPMI 1640, and A673 and HCT116 cells were maintained in DMEM with 4.5 g/l glucose (Thermo Fisher). Media were supplemented with 10% FCS, 2 mM L-glutamine, 100 U/ml penicillin/streptomycin (Thermo Fisher). ES cells were cultivated in collagen-coated (5 μg/cm 2 ; Thermo Fisher) tissue culture asks. Dr. A. Poth (Roßdorf, Germany) kindly provided BALB/c-3T3-A31-1-1 cells from Hatano Research Institute of Japan. BALB/c cells were maintained in DMEM/HAM's F-12 (3.0 g/l glucose; Biochrom) supplemented with 5% FCS and 100 U/ml penicillin/streptomycin. Only subcon uent cells (about 70% con uence) between the passages 20 to 40 were used for the BALB-CTA. All cells were cultivated in a humidi ed incubator at 37°C with 5% CO 2 .
Cells were treated with 0-50 mM AUY922, 1-10 µM VE821, 5-15 µM KU55933 (Selleck chemicals) or their combinations for up to 72 h.
Cells were treated with 0-50 mM AUY922, 1-10 µM VE821, 5-15 µM KU55933 (Selleck chemicals) or their combinations for up to 72 h.
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