Cells were incubated with deoxy-D-glucose-2[1,2-3H(N)] (American Radiolabeled Chemicals, Inc) in Krebs-Ringer-HEPES buffer (NaCl,116 mM; KCl, 4 mM; MgCl2, 1 mM; CaCl2, 1.8 mM; 2-deoxy-D-glucose, 20 mM; HEPES pH 7.4, 10 mM) for 10 min. Cells were then washed, harvested and analyzed for radioactivity using a scintillation counter. A standard was generated to determine the exact molar content in each sample. deoxy-D-glucose-2[1,2-3H(N)] was normalized to protein content as determined by a Bradford Protein Assay (Bio-Rad).
Deoxy d glucose 2 1 2 3h n
Manufactured by American Radiolabeled Chemicals
Deoxy-D-glucose-2[1,2-3H(N)] is a radiolabeled compound used in research applications. It contains a tritium label at the 1st and 2nd carbon positions of the deoxyglucose molecule. This product can be used as a tracer for studying glucose metabolism and transport processes.
Automatically generated - may contain errors
Lab products found in correlation
Bradford protein assay, by Bio-Rad (1 mentions)
Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions)
Human insulin, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Atlanta Biologicals (1 mentions)
Cytochalasin b, by Cayman Chemical (1 mentions)
Actinomycin d, by Cayman Chemical (1 mentions)
Alexa fluor 594 conjugated goat anti mouse antibody, by Jackson ImmunoResearch (1 mentions)
Emetine, by Merck Group (1 mentions)
Calf bovine serum, by Thermo Fisher Scientific (1 mentions)
Hrp conjugated anti mouse and anti rabbit igg, by Cell Signaling Technology (1 mentions)
Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Bodipy 493 503, by Cayman Chemical (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit antibody, by Jackson ImmunoResearch (1 mentions)
2 protocols using deoxy d glucose 2 1 2 3h n
1
Quantifying Cellular Glucose Uptake
2
Insulin Signaling Pathway Visualization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human insulin and emetine were purchased from Sigma-Aldrich (St. Louis, MO); cytochalasin B, BODIPY 493/503, actinomycin D -from Cayman Chemical (Ann Arbor, MI) and fetal bovine serum (FBS) -from Atlanta Biologicals (Lawrenceville, GA). Calf bovine serum (CBS), ProLong® Gold antifade reagent with DAPI, Halt™ Protease and Phosphatase Inhibitor Cocktail, DMEM and DPBS -from Thermo Fisher Scientific (Waltham, MA). Deoxy-D-glucose, 2-[1,2-3H(N)] was purchased from American Radiolabeled Chemicals (St. Louis, MO). Mouse monoclonal antibodies against actin and myc tag, rabbit monoclonal antibody against phospho-TBC1D4 (Thr642), rabbit polyclonal antibodies against the myc tag, phospho-Akt (Ser473 or Thr308), TBC1D4, HRP-conjugated anti-mouse and anti-rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA). Mouse monoclonal anti-syntaxin 6 antibody was purchased from BD Biosciences (Franklin Lakes, NJ). Rabbit polyclonal antibody against TUG were described previously 11, 12 (link) . Alexa Fluor 594-conjugated goat anti-mouse antibody, Alexa Fluor 488conjugated goat anti-rabbit antibody and Alexa Fluor 647-conjugated mouse transferrin were purchased from Jackson ImmunoResearch (West Grove, PA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!