Lung single-cell suspensions were prepared as described before (Khader et al., 2007 (link)), treated with Fc Block (CD16/CD32, 2.4G2, Tonbo Biosciences, San Diego, CA), and stained with appropriate fluorochrome-labeled specific or isotype control antibodies: CD11c (HL3, BD Biosciences), CD11b (M1/70, BD Biosciences and Tonbo Biosciences), Ly6A (D7, eBioscience), CD3 (145–2C11, Tonbo Biosciences), CD4 (GK1.5, BD Biosciences), CD44 (IM7, eBioscience), IFN-γ (XMG1.2, BD Biosciences), CD8 (53–6.7, BD Biosciences), CD69 (H1.2F3, BD Biosciences) and rat IgG1 (BD Biosciences). Cells were processed using a Becton Dickinson FACS LSR Fortessa X20 flow cytometer using FACSDiva software. Cells were gated based on their forward and side scatter characteristics and the frequency of specific cell types was calculated using FlowJo (FlowJo, LLC, Ashland, OR).
Ly6a d7
Manufactured by Thermo Fisher Scientific
Ly6A (D7) is a well-characterized cell surface marker that is widely used in flow cytometry analysis of mouse hematopoietic cells. It is a member of the Ly-6 family of glycosylphosphatidylinositol (GPI)-anchored cell surface proteins.
Automatically generated - may contain errors
Lab products found in correlation
Cd11b m1 70, by BD (2 mentions)
Cd44 im7, by Thermo Fisher Scientific (2 mentions)
Cd11c hl3, by BD (2 mentions)
Cd8 53 6, by BD (2 mentions)
Cd4 gk1, by BD (2 mentions)
Cd69 h1.2f3, by BD (2 mentions)
Facs lsrfortessa x 20 flow cytometer, by BD (2 mentions)
Cd3 145 2c11, by Cytek Biosciences (2 mentions)
Rat igg1, by BD (2 mentions)
Ifn γ xmg1.2, by BD (2 mentions)
2 protocols using ly6a d7
1
Flow Cytometric Analysis of Lung Immune Cells
2
Flow Cytometric Analysis of Lung Immune Cells
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Lung single-cell suspensions were prepared as described before (Khader et al., 2007 (link)), treated with Fc Block (CD16/CD32, 2.4G2, Tonbo Biosciences, San Diego, CA), and stained with appropriate fluorochrome-labeled specific or isotype control antibodies: CD11c (HL3, BD Biosciences), CD11b (M1/70, BD Biosciences and Tonbo Biosciences), Ly6A (D7, eBioscience), CD3 (145–2C11, Tonbo Biosciences), CD4 (GK1.5, BD Biosciences), CD44 (IM7, eBioscience), IFN-γ (XMG1.2, BD Biosciences), CD8 (53–6.7, BD Biosciences), CD69 (H1.2F3, BD Biosciences) and rat IgG1 (BD Biosciences). Cells were processed using a Becton Dickinson FACS LSR Fortessa X20 flow cytometer using FACSDiva software. Cells were gated based on their forward and side scatter characteristics and the frequency of specific cell types was calculated using FlowJo (FlowJo, LLC, Ashland, OR).
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