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2 protocols using ci mmol

1

Radiolabeled Compounds and Drug Compounds Protocol

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[3H]Methyl-4-phenylpyridinium ([3H]MPP+; 80 Ci/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO, USA), while [3H]neurotransmitters (30–50 Ci/mmol) were purchased from Perkin Elmer (Shelton, CT, USA). Methylone, butylone, dibutylone, pentylone, and eutylone were purchased as hydrochloride salts from Cayman Chemical Company (Ann Arbor, MI, USA). All other chemicals and reagents were procured from MilliporeSigma (St. Louis, MO, USA) unless otherwise specified. Cocaine hydrochloride was obtained from the Pharmacy at the National Institute on Drug Abuse (NIDA) Intramural Research Program (IRP). Drugs for the in vivo experiments were dissolved in sterile saline and doses are expressed as the salts.
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2

Radioligand Binding Assay Protocol

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[3H]5-hydroxytryptamine ([3H]5-HT), 80 Ci/mmol; [3H]dihydroalprenolol, levo-[propyl-1,2,3-3H] hydrochloride, 80 Ci/mmol; [3H]paroxetine 20 Ci/mmol; [3H]sertraline, 80 Ci/mmol, and [3H]citalopram, 70 Ci/mmol were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). Unlabeled 5-HT hydrochloride, paroxetine hydrochloride, citalopram hydrobromide, sertraline hydrochloride, fluoxetine hydrochloride, fluvoxamine maleate, and venlafaxine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid assay (BCA assay) reagents were purchased from Thermo Fisher Scientific (Rockford, MA, USA) and Tri Reagent solution was obtained from the Molecular Research Centre (Cincinnati, OH, USA). All other chemicals were of analytical grade.
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