Anti-hemagglutinin mouse monoclonal antibody was purchased from Cell Signaling Technology. Anti-lysosomal associated membrane protein (LAMP) 1 used for immunofluorescence was purchased from Assay Designs. Anti-Rab11a, Anti-Rab5, anti-LAMP1 (used for western blotting) was purchased from AbCam. Anti-Rab5 was purchase from BD Transduction Laboratories. The PIP(3,5) antibody was from Eschelon #ZP035. Alexa-conjugated rabbit and mouse secondary antibodies were purchased from Invitrogen. Anti-PIKfyve was a kind gift from Lois Weisman (University of Michigan). Peroxidase conjugated secondary antibodies were purchased from GE Healthcare Bio-Sciences. All antibodies were characterized before western blotting and immunofluorescence labeling studies by serial dilutions to determine optimal conditions and negative controls (usually secondary antibody alone) to ensure specificity (data not shown).
Alexa conjugated rabbit and mouse secondary antibodies
Manufactured by Thermo Fisher Scientific
Alexa-conjugated rabbit and mouse secondary antibodies are fluorescently labeled secondary antibodies used for detection in immunoassays and other fluorescent-based applications. They bind to primary antibodies raised in rabbit or mouse and emit fluorescence when excited, allowing visualization and quantification of target proteins or other biomolecules.
Automatically generated - may contain errors
2 protocols using alexa conjugated rabbit and mouse secondary antibodies
1
Antibody Characterization for Western Blotting and Immunofluorescence
2
Antibody Characterization for Western Blotting and Immunofluorescence
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Anti-hemagglutinin (HA) mouse monoclonal antibody was purchased from Cell Signaling Technology (Beverly, MA). Anti–lysosomal-associated membrane protein 1 (LAMP1) used for immunofluorescence was purchased from Assay Designs (Ann Arbor, MI). Anti-Rab11a, anti-Rab5, and anti-LAMP1 (used for Western blotting) were purchased from AbCam (Cambridge, MA). anti-Rab5 was purchase from BD Transduction Laboratories (Lexington, KY). The PIP(3,5) antibody (cat. no. ZP035) was from Eschelon Biosciences (Salt Lake City, UT). Alexa-conjugated rabbit and mouse secondary antibodies were purchased from Invitrogen/Life Technologies (Carlsbad, CA). Anti-PIKfyve was a kind gift from Lois Weisman (University of Michigan). Peroxidase conjugated secondary antibodies were purchased from GE Healthcare Bio-Sciences (Pittsburgh, PA). All antibodies were characterized before western blotting and immunofluorescence labeling studies by serial dilutions to determine optimal conditions and negative controls (usually secondary antibody alone) to ensure specificity (data not shown).
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