Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti sirt1 polyclonal antibody

Manufactured by Santa Cruz Biotechnology
Visit Supplier
Sourced in United States

The Rabbit anti-SIRT1 polyclonal antibody is a laboratory reagent used for the detection and identification of the SIRT1 protein in various biological samples. This antibody is produced in rabbits and recognizes the SIRT1 protein, which is a member of the sirtuin family of proteins involved in cellular processes such as metabolism, aging, and stress response.

Automatically generated - may contain errors

Lab products found in correlation

Benchmark xt, by Roche (2 mentions) Rabbit anti β actin polyclonal antibody, by Merck Group (2 mentions) Cell disruption buffer, by Thermo Fisher Scientific (1 mentions) Rabbit anti gapdh antibody, by GeneTex (1 mentions) Non fat milk, by Euroclone (1 mentions) Complete and phosstop, by Roche (1 mentions) Liteablot turbo, by Euroclone (1 mentions) Nicotinamide, by Merck Group (1 mentions) Sirtinol, by Merck Group (1 mentions) Sa β galactosidase staining kit, by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated igg, by Santa Cruz Biotechnology (1 mentions) β galactosidase enzyme assay kit, by Promega (1 mentions) Ang 2, by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Resveratrol, by Merck Group (1 mentions) Luciferase assay system, by Promega (1 mentions) Goat anti rabbit polyclonal antibody, by Santa Cruz Biotechnology (1 mentions)

4 protocols using rabbit anti sirt1 polyclonal antibody

1

Acetylation Analysis of p53 Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in Cell Disruption Buffer (Ambion) containing inhibitors of proteases and phosphatases (Complete and PhosSTOP, Roche); samples to be analyzed for p53 acetylation were supplemented with 10 mM nicotinamide (Sigma-Aldrich) to inhibit deacetylases. Samples were analyzed with a Bradford protein assay (Bradford, 1976 (link)), balanced for total protein and subjected to SDS-PAGE followed by electrotransfer to nitrocellulose membrane (GE Healthcare). Blots were saturated in non-fat milk (Euroclone) and incubated with rabbit anti-GAPDH antibody (Genetex), rabbit anti-β-actin polyclonal antibody (Sigma-Aldrich), rabbit anti-acetylated p53 antibody (Lysine-382, Cell Signaling), goat anti-p53 polyclonal antibody, and rabbit anti-SIRT1 polyclonal antibody (both from Santa Cruz Biotechnology) followed by horseradish peroxidase-conjugated secondary antibodies (Pierce or GE Healthcare). Immunoreactive bands were detected using Femto (Pierce) or LiteAblot Turbo (Euroclone) chemiluminescence reagent and a digital imager (BioRad ChemiDoc XRS or Cambridge UVTEC).
Sharma V.K., Raimondi V., Ruggero K., Pise-Masison C.A., Cavallari I., Silic-Benussi M., Ciminale V, & D’Agostino D.M. (2018). Expression of miR-34a in T-Cells Infected by Human T-Lymphotropic Virus 1. Frontiers in Microbiology, 9, 832.
+ Open protocol
+ Expand
2

Resveratrol Modulation of SIRT1 in Cell Senescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ang II, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), polyclonal rabbit anti-β-actin antibody, resveratrol, SA β-galactosidase staining kit, sirtinol, and whey protein (W1500) were obtained from Sigma-Aldrich Co. (USA). The β-galactosidase enzyme assay kit and luciferase assay system were obtained from Promega (USA). Rabbit anti-SIRT1 polyclonal antibody and horseradish peroxidase (HRP)-conjugated IgG were obtained from Santa Cruz Biotechnology (USA).
Hwang J.S., Han S.G., Lee C.H, & Seo H.G. (2017). Whey Protein Attenuates Angiotensin II-Primed Premature Senescence of Vascular Smooth Muscle Cells through Upregulation of SIRT1. Korean Journal for Food Science of Animal Resources, 37(6), 917-925.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of Oral Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
IHC was conducted to detect protein expression in paraffin-embedded oral squamous cell carcinoma specimens. The slides were stained with rabbit anti-SIRT1 polyclonal antibody (1:25 Santa Cruz Biotechnology, Santa Cruz, CA, USA) and goat anti-Smad4 polyclonal antibody (1:100, Santa Cruz Biotechnology) using an automatic slide stainer BenchMark XT (Ventana Medical Systems; Tucson AZ, USA). Hematoxylin was used as the counterstain. Two independent pathologists evaluated each slide under a light microscope. Immunoreactivity was classified by estimating the percentage (P) of tumor cells exhibiting characteristic staining (from an undetectable level, 0%, to homogeneous staining, 100%) and by estimating the intensity (I) of staining (1, weak staining; 2, moderate staining; 3, strong staining). Results were scored by multiplying the percentage of positive cells by the intensity (i.e. quick score Q = P x I; maximum = 300) [98 (link)].
Chen I.C., Chiang W.F., Huang H.H., Chen P.F., Shen Y.Y, & Chiang H.C. (2014). Role of SIRT1 in regulation of epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis. Molecular Cancer, 13, 254.
+ Open protocol
+ Expand
4

Quantifying SIRT1 Expression in OSCC

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemical staining was performed to detect protein localization and expression in paraffin-embedded OSCC specimens. The sections were stained with rabbit anti-SIRT1 polyclonal antibody (1:1000; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and goat anti-rabbit polyclonal antibody (1:5000; Santa Cruz Biotechnology), using an automatic slide stainer BenchMark XT (Ventana Medical Systems, Tucson, AZ, USA). Hematoxylin was used as the counterstain. Sections were evaluated using a microscope (Nikon, Tokyo, Japan) by one of the authors.
At higher magnification (×400), five visual fields were selected randomly, the expression positive signal was analyzed using Image-proplus software. SIRT1 protein levels in OSCC tissue and adjacent normal epithelium specimens were compared in accordance with the integral optical density (IOD) as a parameter for semiquantitative detection.
Kang Y.Y., Sun F.L., Zhang Y, & Wang Z. (2018). SIRT1 acts as a potential tumor suppressor in oral squamous cell carcinoma. Journal of the Chinese Medical Association : JCMA, 81(5).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!