Agilent 1200 device

Echocardiography was conducted using a Vivid E9 ultrasound system (GE Healthcare, Milwaukee, WI, USA). The main parameters used in the present study included FS, LVEF, LVMI, left ventricular end-diastolic diameter, interventricular septal thickness and left ventricular posterior wall thickness.
After overnight fasting, the peripheral venous blood of patients was collected using a vacutainer tube (without any anticoagulant) and an EDTA-coated tube between 8:00 am and 9:00 am. Serum samples were obtained by centrifuging the collected samples at 3500 rpm for 10 min at 4 °C, and plasma samples were obtained by centrifuging the collected samples at 2000 × g for 10 min at 4 °C. All blood samples were stored at −80 °C until further use.
HbA1c levels of whole blood samples were measured using high-performance liquid chromatography (Agilent 1200 device, Agilent Technologies, Waldbronn, Germany). Serum TC, TG, LDL-C, HDL-C, FBG, AST and ALT levels were determined using a biochemical autoanalyser (UniCel DxC 600 Synchron, Beckman Coulter, California, United States). NT-proBNP in the plasma was measured using an electrochemiluminescence immunoassay (ProBNP Elecsys®, Roche Diagnostics GmbH).

Glyphosate was analyzed using an Agilent 1200 device (Agilent Technologies International Japan) coupled to an AB Sciex TripleTOF 5600+ (AB Sciex, Framingham). Chromatographic separation was performed with hydrophilic interaction liquid chromatography (HILIC) column (InertSustain Amide, 100 mm × 3.0 mm i. d., 3 μm; GL Sciences Inc.). The mobile phase was 0.1% formic acid–acetonitrile (80:20, v/v) at 0.2 mL/min and the injection volume was 2 μL. The column was kept at 40 °C in the column oven. An AB Sciex 5600+ triple quadrupole mass spectrometer was used for mass spectrometry determination. The software AB Sciex Analyst was used for data collection. Ionization was performed in negative mode. Mass spectrometry data were collected in the multiple reaction monitoring (MRM) mode. The MRM parameters for the target analytes are shown in Supplementary Data 2. Instrument settings for ESI analysis were as follows: Heater temperature, 350 °C; curtain gas, 25 psi; nebulizer gas, 50 psi; heater gas, 50 psi; ionspray voltage, −4500 V; dwell time, 250 ms.