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Mannan

Manufactured by Megazyme
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Sourced in Ireland

Mannan is a lab equipment product offered by Megazyme. It is a polysaccharide composed of mannose units. The core function of Mannan is to serve as a standard for the quantitative analysis of mannose-containing polysaccharides.

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Lab products found in correlation

Glucomannan, by Megazyme (2 mentions) Curdlan, by Megazyme (2 mentions) Microcrystalline cellulose, by Thermo Fisher Scientific (2 mentions) Formic acid, by Thermo Fisher Scientific (2 mentions) Glacial acetic acid, by Merck Group (2 mentions) Sodium acetate, by Merck Group (2 mentions) Galactomannan, by Megazyme (2 mentions) β glucan, by Megazyme (2 mentions) Galactan, by Megazyme (2 mentions) Arabinan, by Megazyme (2 mentions) Xyloglucan, by Megazyme (2 mentions) Arabinoxylan, by Megazyme (2 mentions) Lichenan, by Megazyme (2 mentions) Xylan, by Megazyme (2 mentions) Amylopectin, by Biosynth (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Sodium borohydride, by Merck Group (1 mentions) Hplc grade acetonitrile, by Honeywell (1 mentions) Arabinogalactan, by Megazyme (1 mentions) Hplc grade acetonitrile acn, by Honeywell (1 mentions)

2 protocols using mannan

1

Polysaccharide Characterization Protocol

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Sodium acetate, hydrogen peroxide (H2O2), sodium hydroxide (NaOH), sodium borohydride (NaBH4), iron(III) sulfate pentahydrate (Fe2(SO4)3), and glacial acetic acid were purchased from Sigma-Aldrich (St. Louis, MO). Amylopectin was obtained from Carbosynth (Compton, UK). Amylose, xyloglucan, arabinoxylan, xylan, glucomannan, galactomannan, mannan, curdlan, galactan, β-glucan, arabinan, and lichenan were purchased from Megazyme (Bray, Ireland). Microcrystalline cellulose was purchased from ACROS Organics. Formic Acid (FA) was purchased from Fisher Scientific (Belgium, UK). Acetonitrile (HPLC grade) was purchased from Honeywell (Muskegon, MI). Nano-pure water was used for all experiments.
Amicucci M.J., Nandita E., Galermo A.G., Castillo J.J., Chen S., Park D., Smilowitz J.T., German J.B., Mills D.A, & Lebrilla C.B. (2020). A nonenzymatic method for cleaving polysaccharides to yield oligosaccharides for structural analysis. Nature Communications, 11, 3963.
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2

Extraction and Analysis of Plant-Derived Glycans

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Sodium borohydride (NaBH4), sodium hydroxide (NaOH), sodium acetate, glacial acetic acid, trifluoroacetic acid (TFA), hydrogen peroxide (H2O2), and iron(III) sulfate pentahydrate (Fe2(SO4)3·5H2O) were purchased from Sigma-Aldrich (St. Louis, MO). Galactan, amylose, β-glucan, arabinan, xyloglucan, curdlan, arabinoxylan, lichenan, glucomannan, mannan, galactomannan, arabinoGalactan and xylan were purchased from Megazyme (Bray, Ireland). Microcrystalline cellulose was purchased from ACROS Organics. Yellow corn meal (Zea mays), wheat grass (Triticum sp.), whole grain oat cereal (Avena sativa), horseradish root (Armoracia rusticana), and coffee grounds (Coffea arabica) were purchased from the Davis Co-op (Davis, CA). Coconut (Cocos nucifera), jackfruit (Artocarpus heterophyllus), guava (Psidium guajava), yam leaves (Dioscorea sp.), bok choy leaves (Brassica rapa) were purchased from 99 Ranch Market (Sacramento, CA). Acetonitrile (ACN, HPLC grade) was purchased from Honeywell (Muskegon, MI). Formic Acid (FA) was purchased from Fisher Scientific (Belgium, UK). Porous graphitized carbon (PGC) solid phase extraction (SPE) plates were purchased from Glygen (Columbia, MD). Nanopure water (18.2 MΩ-cm) was used for all experiments.
Nandita E., Bacalzo NP J.r., Ranque C.L., Amicucci M.J., Galermo A, & Lebrilla C.B. (2021). Polysaccharide Identification Through Oligosaccharide Fingerprinting. Carbohydrate polymers, 257, 117570.
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