Singlet oxygen production was calculated using the commercially available Singlet Oxygen Sensor Green (SOSG) kit (Invitrogen). The SOSG was dissolved in methanol and the working solution was prepared in ultrapure water. Free BPD, BPD-PFP, ICG-PFP and BPD+ICG-PFP (with different loading ratios of BPD and ICG) nanodroplet solutions were diluted to a concentration of 5 µM in phosphate-buffered saline (PBS) supplemented with 10% FBS. The SOSG was added to the solutions at a concentration of 5 µM and the solutions were irradiated with a 690 nm laser at an irradiance of 150 mW/cm2 and a total light dose of 20 J/cm2. After irradiation, the SOSG fluorescence intensity was recorded using the Spectramax M5 plate reader using 460 nm excitation with a 515 nm cut-off filter and emission at 525 nm. For testing the efficiency of oxygen in carrying BPD-PFP nanodroplets to produce singlet oxygen in hypoxic conditions, nitrogen gas was flushed through the environment for 5 min to reduce the oxygen concentration. Following this, the solution was irradiated and SOSG fluorescence was measured.
Singlet oxygen sensor green kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Singlet Oxygen Sensor Green (SOSG) kit is a fluorescent probe designed to detect and quantify the presence of singlet oxygen in biological systems. The kit provides a sensitive and specific way to measure this reactive oxygen species.
Automatically generated - may contain errors
Lab products found in correlation
1 3 diphenylisobenzofuran dpbf, by Merck Group (2 mentions)
Dopamine hydrochloride, by Merck Group (2 mentions)
Paraformaldehyde (pfa), by Boster Bio (1 mentions)
4 nitroquinoline 1 oxide 4nqo, by Merck Group (1 mentions)
β actin monoclonal antibody, by Proteintech (1 mentions)
Cell counting kit 8 cck 8, by Selleck Chemicals (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Ros assay kit, by Beyotime (1 mentions)
Cal27, by American Type Culture Collection (1 mentions)
Hacat cell line, by American Type Culture Collection (1 mentions)
Crl 1435, by American Type Culture Collection (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Sodium tripolyphosphate, by Merck Group (1 mentions)
Low molecular weight chitosan, by Merck Group (1 mentions)
Glacial acetic acid, by Chem-Supply (1 mentions)
Rose bengal, by Merck Group (1 mentions)
Dimethylformamide dmf, by Merck Group (1 mentions)
Chlorpromazine, by Merck Group (1 mentions)
Aqueous ammonia solution, by Merck Group (1 mentions)
Genistein, by Merck Group (1 mentions)
6 protocols using singlet oxygen sensor green kit
1
Singlet Oxygen Production Quantification
2
Photodynamic Therapy Using ITIC-Th Nanoparticles
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ITIC-Th was purchased from Derthon (Shenzhen, China). Tetrahydrofuran (THF) and dichloromethane were purchased from Admas-Beta (Shanghai, China). DSPE-mPEG 2000 was purchased from ToYongBio Tech (Shanghai, China). Leuk-1 was kindly provided by Professor Li Mao of the University of Maryland Dental School (Baltimore, USA). CAL 27 and HaCaT cell lines were get from the American Type Culture Collection (ATCC; Manassas, VA, USA). Singlet Oxygen Sensor Green (SOSG) kit was from Invitrogen (Thermo Fisher Scientific, USA). 1, 3-diphenylisobenzofuran (DPBF) and 4-nitroquinoline 1-oxide (4NQO) were purchased from Sigma-Aldrich (Saint Louis, USA). Cell Counting Kit-8 (CCK-8) was purchased from Bimake (Shanghai, China). ROS assay kit and DAPI were obtained from Beyotime (Shanghai, China). Cyclin D1 Monoclonal Antibody, p53 Monoclonal Antibody, Aldehyde dehydrogenase isoform 1 (ALDH1) Polyclonal Antibody, Programmed cell death 5 (PDCD5) Polyclonal antibody, and β-Actin Monoclonal Antibody were purchased from Proteintech (Wuhan, China). The anti-podoplanin (PDPN) antibody was purchased from Abcam (Shanghai, China). 5-ALA was purchased from FUDAN-ZHANGJIANG (Shanghai, China). Paraformaldehyde was from BOSTER (Wuhan, China). The Super -maxvision mouse/rabbit universal HRP Kit was from Typing Biotech (Nanjing, China).
3
Photosensitizer Mediated Cancer Cell Assay
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PPIX (P8293) was purchased from Sigma Aldrich (Oakville, ON, CA). Prostate cancer (PC3) cell line (CRL-1435) was purchased from ATCC (Manassas, VA, USA). Singlet oxygen sensor green (SOSG) kit was purchased from ThermoFisher, MA, USA. Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Life Technologies, Carlsbad, CA, USA.
4
Photodynamic Therapy Nanoparticle Synthesis
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Rose Bengal (4,5,6,7-Tetrachloro-2′,4′,5′,7′-tetraiodofluorescein disodium salt), low molecular weight chitosan (MW = 50–190 kDa, deacetylation degree~75%) and sodium tripolyphosphate (TPP) were purchased from Sigma-Aldrich (Sydney, NSW, Australia). Glacial acetic acid was sourced from Chem-Supply (Gillman, SA, Australia). NaOH pellets were purchased from PanReac ApplyChem (Barcelona, Spain). 1,3-diphenylisobenzofuran (DPBF) and dimethylformamide (DMF) were purchased from Sigma-Aldrich (Sydney, NSW, Australia). The singlet Oxygen Sensor Green (SOSG) kit was purchased from Thermo Fisher (Sydney, NSW, Australia). All chemicals and reagents were of the highest purity grade commercially available. Deionised water (18.2 MΩ, 25 °C) was collected from a Milli-Q Advantage A10 water purification system and used to make sample solutions.
5
Photodynamic Therapy for Breast Cancer
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Dopamine hydrochloride, ammonia aqueous solution (NH4OH, 28–30% wt%), Chlorin e6 (Ce6) and cell uptake inhibitors (nocodazole, cytochalasin D, chlorpromazine, genistein, and β-cyclodextran) were purchased from Sigma-Aldrich (Shanghai, China). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was provided by KeyGEN Biotech. Co., Ltd. (Jiangsu, China). Amine-PEG-thiol (NH2-PEG-SH, MW ≈ 2000 Da) was obtained from Laysan Bio. Inc. (Shanghai, China). Singlet oxygen sensor green (SOSG) kits were purchased from Invitrogen (Shanghai, China). Human breast tumor cells (SKBR-3) and human mammary epithelial cells (MCF 10A) were purchased from ATCC (Manassas, VA). Fetal bovine serum (FBS), trypsin, media and phosphate buffered saline (PBS) were purchased from Gibco/Life Technologies (Shanghai, China).
6
Immunogenic Cell Death Protocol
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Dopamine hydrochloride, ammonia aqueous solution (NH4OH, 28–30 wt%), ethanol, pluronic F127, 1, 3, 5-trimethylbenzene, and IR-780 were purchased from Sigma-Aldrich (Shanghai, China). Mouse breast cancer cells (4T1) were purchased from American Type Culture Collection (ATCC, Manassas, VA). CD3, CD4, CD8 flow cytometric antibodies and their homotypic antibodies were obtained from BD Biosciences (Shanghai, China). Anti-calreticulin (CRT) antibody was obtained from Abcam (Shanghai, China). The enzyme linked immunosorbent assay (ELISA) kits for the detection of adenosine triphosphate (ATP), high-mobility group box 1 (HMGB1), tumor necrosis factor (TNF-α), interferon γ (IFN-γ), interleukin-2 (IL-2), and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were obtained from KeyGEN Biotech. Co., Ltd. (Jiangsu, China). Tumor dissociation kit of mouse was purchased from Miltenyi Biotec (Germany). Fetal bovine serum (FBS), trypsin, Roswell Park Memorial Institute 1640 (RPMI 1640) culture medium, and phosphate buffer saline (PBS) were purchased from Gibco/Life Technologies (Shanghai, China). Singlet oxygen sensor green (SOSG) kits were purchased from Invitrogen (Shanghai, China).
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