Opaque white plates

Manufactured by Thermo Fisher Scientific

Sourced in United States

Opaque white plates are a type of laboratory equipment designed for various applications. They feature an opaque white surface that helps to minimize light interference and improve the accuracy of optical measurements. These plates are commonly used in a range of laboratory settings, including cell culture, assay development, and high-throughput screening.

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Lab products found in correlation

Synergy ht, by Agilent Technologies (2 mentions) Celltiter glo, by Promega (2 mentions)

Spelling variants of this product

White opaque 96-well microtiter plates (3 citations) Nunc F96 MicroWell white opaque polystyrene plates (2 citations) White opaque microtitre plates (2 citations) Opaque white 96-well plates (2 citations) Pierce white opaque 96-well plates (2 citations) 96-well opaque white plate (2 citations)

2 protocols using opaque white plates

ATP-based Cytotoxicity Assay in Vero Cells

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Cytotoxicity of compounds was assessed by ATP detection using a commercially available kit, as per manufacturer instruction (CellTiter-Glo, Promega, Madison, WI, USA). Briefly, 10⁴ Vero cells were seeded per well in a 96-well plate using 100 µL of complete medium. After 18 h, medium was replaced with several concentrations of compounds in maintenance medium and incubated further for 48 h. Then, 100 µL of freshly reconstituted ATP detection reagent was added to each well and the plate carefully agitated for 2 min; cell lysates were transferred to opaque white plates (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. 236105) and the luminescence signal was detected after 10 min using a multidetection microplate reader (Synergy HT, Biotek, Winooski, VT, USA). Cytotoxicity was expressed as the reduction of luminescent signal in compound-treated cells as compared to nontreated cells.

de la Guardia C., Stephens D.E., Dang H.T., Quijada M., Larionov O.V, & Lleonart R. (2018). Antiviral Activity of Novel Quinoline Derivatives against Dengue Virus Serotype 2. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(3), 672.

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Cytotoxicity Assessment of Compounds

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Cytotoxicity of compounds was assessed by ATP detection using a commercially available kit, as per manufacturer instruction (CellTiter-Glo, Promega, State abbrev., USA). Briefly, 10⁴ Vero cells were seeded per well in a 96-well plate using 100 μL of complete medium. After 18 h, medium was replaced with several concentrations of compounds in maintenance medium and incubated further for 48 h. Then, 100 μL of freshly reconstituted ATP detection reagent was added to each well and the plate carefully agitated for 2 min; cell lysates were transferred to opaque white plates (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. 236105) and the luminescence signal was detected after 10 min using a multidetection microplate reader (Synergy HT, Biotek, Winooski, VT, USA). Cytotoxicity was expressed as the reduction of luminescent signal in compound-treated cells as compared to nontreated cells.

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