The following primary antibodies were used: anti‐p53 (Abcam, UK), anti‐p16INK4a (Abcam, UK), anti‐p16INK4a (Abcam, UK), anti‐p21CIP1/WAF1 (Abcam, UK), anti‐GATA4 (Santa‐Cruz, USA), anti‐GATA4 (Abcam, UK), anti‐CCN1 (Abcam, UK), anti‐γ‐H2AX (Abcam, UK), anti‐γ‐H2AX (CST, USA), anti‐GAPDH (Proteintech, China), anti‐β‐actin (Boster, China), anti‐interleukin (IL)‐1α (Proteintech, China), anti–monocyte chemotactic protein‐1 (Abcam, UK), anti–tumor necrosis factor‐α (TNF‐α) (Abcam, UK), and anti‐α‐actin (Proteintech, China). All secondary antibodies were from ZSGB‐BIO Company (China). Reagents wheat germ agglutinin and Alda‐1 were purchased from Sigma‐Aldrich, USA. All of the catalog numbers of the antibodies are listed in Table S1 .
Anti ccn1
Manufactured by Abcam
Sourced in United Kingdom
Anti-CCN1 is a protein that plays a role in cell adhesion, proliferation, and migration. It is a member of the CCN family of proteins and is involved in various cellular processes. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Wheat germ agglutinin (wga), by Merck Group (1 mentions)
Alda 1, by Merck Group (1 mentions)
Anti β actin, by Boster Bio (1 mentions)
Anti gata4, by Abcam (1 mentions)
Anti p16ink4a, by Abcam (1 mentions)
Anti α actin, by Proteintech (1 mentions)
Anti tumor necrosis factor α tnf α, by Abcam (1 mentions)
Anti p21cip1 waf1, by Abcam (1 mentions)
Anti p53, by Abcam (1 mentions)
Anti γh2ax, by Abcam (1 mentions)
Anti gata4, by Santa Cruz Biotechnology (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Odyssey imaging system, by LI COR (1 mentions)
Anti actin, by Merck Group (1 mentions)
Nupage bis tris gel, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
M per mammalian protein extraction reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using anti ccn1
1
Detailed Antibody Characterization Protocol
2
Quantifying Lung Protein Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Prior to protein separation, lung homogenates were lysed with M-PER mammalian protein extraction reagent (Thermo Scientific) and BAL was concentrated using Amicon Ultra-0.5 mL centrifugal filters with a 3 KDa cut off. Equal amounts of protein (lung) or equal volume (BAL) were were separated on 4–12% bis-Tris NuPAGE gels (Invitrogen), transferred to PVDF membranes (ThermoFisher Scientific). Immunodetection was performed using anti-CCN1 (abcam) and anti-actin (Sigma) antibodies. Proteins were visualized and quantified on with the Odyssey imaging system (Li-COR). Data were normalized to the mean intensity of the protein bands in the c-IgG (in vivo) or ATH35L (in vitro) groups.
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