Scaffolds were fixed in 10% formalin, paraffin-embedded, and sectioned at 4 μm. Following deparaffinization, the sections were treated with 0.5% Triton X-100 (MP Biomedicals, LLC, Santa Ana, CA), blocked with 10% normal goat serum (Jackson ImmunoResearch Laboratories, Inc, West Grove, PA) for 1 hour, and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C, for 20 minutes. Sections were then separately incubated with anti-Yap (1:200, Santa Cruz Biotechnology, Santa Cruz, CA) anti-non-p-β-catenin (1:1000 Cell Signaling Technologies, Beverly, MA) overnight at 4 °C. After washing, sections were incubated in anti-rabbit IgG Alex Fluor Plus 488 or anti-mouse IgG Alexa Fluor Plus 594 (ThermoFisher, Waltham, MA). Coverslips were mounted with Prolong Gold Antifade Reagent with Dapi (Cell Signaling Technologies, Danvers, MA). Images were captured with the Zeis LSM900 confocal laser scanning microscope with Zen 3.1 Blue software (Zeiss, Oberkochen, Germany).
Anti rabbit igg alex fluor plus 488
Manufactured by Thermo Fisher Scientific
The Anti-rabbit IgG Alexa Fluor Plus 488 is a fluorescent secondary antibody used to detect and visualize rabbit primary antibodies in various immunodetection techniques. It is conjugated with a bright, photostable Alexa Fluor Plus 488 fluorescent dye, which emits green fluorescence when excited.
Automatically generated - may contain errors
Lab products found in correlation
Sodium citrate buffer, by Thermo Fisher Scientific (2 mentions)
Normal goat serum (ngs), by Jackson ImmunoResearch (2 mentions)
Anti mouse igg alexa fluor plus 594, by Thermo Fisher Scientific (2 mentions)
Zeis lsm900 confocal laser scanning microscope, by Zeiss (2 mentions)
Zen 3.1 blue software, by Zeiss (2 mentions)
Anti yap, by Santa Cruz Biotechnology (2 mentions)
Triton x 100, by MP Biomedicals (2 mentions)
Anti non p β catenin, by Cell Signaling Technology (1 mentions)
Prolong gold antifade reagent with dapi, by Cell Signaling Technology (1 mentions)
2 protocols using anti rabbit igg alex fluor plus 488
1
Immunohistochemical Analysis of Yap and β-Catenin
2
Immunohistochemical Analysis of Yap and β-Catenin
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Scaffolds were fixed in 10% formalin, paraffin-embedded, and sectioned at 4 µm.
Following deparaffinization, the sections were treated with 0.5% Triton X-100 (MP Biomedicals, LLC, Santa Ana, CA), blocked with 10% normal goat serum (Jackson ImmunoResearch Laboratories, Inc, West Grove, PA) for 1 hour, and subjected to heatinduced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C, for 20 minutes. Sections were then separately incubated with anti-Yap (1:200, Santa Cruz Biotechnology, Santa Cruz, CA) anti-non-p-b-catenin (1:1000 Cell Signaling Technologies, Beverly, MA) overnight at 4 °C. After washing, sections were incubated in anti-rabbit IgG Alex Fluor Plus 488 or anti-mouse IgG Alexa Fluor Plus 594 (ThermoFisher, Waltham, MA). Coverslips were mounted with Prolong (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Gold Antifade Reagent with Dapi (Cell Signaling Technologies, Danvers, MA). Images were captured with the Zeis LSM900 confocal laser scanning microscope with Zen 3.1 Blue software (Zeiss, Oberkochen, Germany).
Following deparaffinization, the sections were treated with 0.5% Triton X-100 (MP Biomedicals, LLC, Santa Ana, CA), blocked with 10% normal goat serum (Jackson ImmunoResearch Laboratories, Inc, West Grove, PA) for 1 hour, and subjected to heatinduced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C, for 20 minutes. Sections were then separately incubated with anti-Yap (1:200, Santa Cruz Biotechnology, Santa Cruz, CA) anti-non-p-b-catenin (1:1000 Cell Signaling Technologies, Beverly, MA) overnight at 4 °C. After washing, sections were incubated in anti-rabbit IgG Alex Fluor Plus 488 or anti-mouse IgG Alexa Fluor Plus 594 (ThermoFisher, Waltham, MA). Coverslips were mounted with Prolong (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Gold Antifade Reagent with Dapi (Cell Signaling Technologies, Danvers, MA). Images were captured with the Zeis LSM900 confocal laser scanning microscope with Zen 3.1 Blue software (Zeiss, Oberkochen, Germany).
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