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Pcag ires egfp

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PCAG-IRES-EGFP is a plasmid vector designed for the expression of two genes from a single bicistronic mRNA. The vector contains an internal ribosome entry site (IRES) sequence that allows for the translation of a second gene located downstream of the IRES. The EGFP (enhanced green fluorescent protein) gene is included as a reporter for monitoring gene expression.

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Lab products found in correlation

Fast green, by Merck Group (2 mentions) Standard forceps type circular electrodes, by Nepa Gene (2 mentions) Cuy21edit, by Nepa Gene (2 mentions)

2 protocols using pcag ires egfp

1

Tripolar In Utero Electroporation of Visual Cortex

Cited 1 time
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Tripolar in utero electroporation of the visual cortex was performed as previously 52 (link). Timed-pregnant Long Evans rats were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (pCAG-IRES-EGFP, 1.5 μg/μL in water) and dye Fast Green (0.3 μg/μL; Sigma, St. Louis, MO) were injected (5–6 μL) through the uterine wall into one of the embryos’ lateral ventricles by a 30-G needle. While the embryo’s head was carefully held between standard forceps-type circular electrodes (10 mm diameter; positive poles; Nepa Gene, Chiba, Japan), a third electrode (7×6×1 mm, negative pole) was positioned on the back of the embryo’s head. 5 electrical pulses (amplitude, 50V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Deidda G., Allegra M., Cerri C., Naskar S., Bony G., Zunino G., Bozzi Y., Caleo M, & Cancedda L. (2014). Early depolarizing GABA controls critical period plasticity in the rat visual cortex. Nature neuroscience, 18(1), 87-96.
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2

Tripolar In Utero Electroporation of Visual Cortex

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tripolar in utero electroporation of the visual cortex was performed as previously 52 (link). Timed-pregnant Long Evans rats were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (pCAG-IRES-EGFP, 1.5 μg/μL in water) and dye Fast Green (0.3 μg/μL; Sigma, St. Louis, MO) were injected (5–6 μL) through the uterine wall into one of the embryos’ lateral ventricles by a 30-G needle. While the embryo’s head was carefully held between standard forceps-type circular electrodes (10 mm diameter; positive poles; Nepa Gene, Chiba, Japan), a third electrode (7×6×1 mm, negative pole) was positioned on the back of the embryo’s head. 5 electrical pulses (amplitude, 50V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Deidda G., Allegra M., Cerri C., Naskar S., Bony G., Zunino G., Bozzi Y., Caleo M, & Cancedda L. (2014). Early depolarizing GABA controls critical period plasticity in the rat visual cortex. Nature neuroscience, 18(1), 87-96.
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