Perchloric acid

Manufactured by AppliChem

Sourced in Germany

Perchloric acid is a chemical compound with the formula HClO4. It is a strong oxidizing agent and is commonly used in various laboratory applications.

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Lab products found in correlation

2 protocols using perchloric acid

Metabolic Inhibitor Procurement and Usage

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2-Deoxy-d-glucose (2DG), antimycin A, BAM15, fetal calf serum (FCS), oligomycin and rotenone were purchased from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). Bovine serum albumin, dimethyl sulfoxide (DMSO), perchloric acid, NAD + , NADH and NADP + were from AppliChem (Darmstadt, Germany; RRID:SCR_005814). β-lapachone was from Abcam (Berlin, Germany; RRID:SCR_012931) and WST1 from Dojindo (Munich, Germany). Etomoxir and UK5099 were obtained from Merck (Darmstadt, Germany; RRID:SCR_001287). Dulbecco's modified Eagles medium (DMEM) and penicillin G/streptomycin sulfate solution were obtained from Thermo Fisher Scientific (Schwerte, Germany; RRID:SCR_008452). The Cell Titer Glo® 2.0 ATP Assay Kit was obtained from Promega (Walldorf, Germany; RRID:SCR_006724). ATP and the enzymes used for assays to quantify lactate, glucose, 2DG and 2DG6P were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Carl Roth (Karlsruhe, Germany; RRID:SCR_005711), Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland). Sterile cell culture materials as well as transparent and black microtiter plates were obtained from Sarstedt (Nümbrecht, Germany).

Harders A.R., Watermann P., Karger G., Denieffe S.C., Weller A., Dannemann A.C., Willker J.E., Köhler Y., Arend C, & Dringen R. (2024). Consequences of a 2-Deoxyglucose Exposure on the ATP Content and the Cytosolic Glucose Metabolism of Cultured Primary Rat Astrocytes. Neurochemical research.

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ATP Quantification in Cell Cultures

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Sterile cell culture materials, unsterile 96-well plates and black microtiter plates were purchased from Sarstedt (Nümbrecht, Germany). Fetal calf serum (FCS), adenylate kinase, AMP, antimycin A, creatine, creatine kinase and 2-deoxyglucose (2DG) were obtained from Sigma-Aldrich (Steinheim, Germany; RRID:SCR_008988). ADP, Dulbecco’s modified Eagles medium (DMEM with 25 mM glucose, catalog number 52100-021) and penicillin G/streptomycin sulfate solution were from Thermo Fisher Scientific (Schwerte, Germany). The Cell Titer Glo^® 2.0 ATP Assay Kit was purchased from Promega (Walldorf, Germany; RRID:SCR_006724). Bovine serum albumin, dimethyl sulfoxide (DMSO) and perchloric acid were from AppliChem (Darmstadt, Germany; RRID:SRC_005814). ATP and pyruvate kinase were purchased from Roche Diagnostics (Mannheim, Germany; RRID:SCR_001326). All other basal chemicals were obtained from Sigma-Aldrich (Steinheim, Germany), Roth (Karlsruhe, Germany), Riedel-de Haën (Seelze, Germany) or Fluka (Buchs, Switzerland).

Karger G., Berger J, & Dringen R. (2023). Modulation of Cellular Levels of Adenosine Phosphates and Creatine Phosphate in Cultured Primary Astrocytes. Neurochemical Research, 49(2), 402-414.

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