Fac scount reagents

Manufactured by BD

Sourced in United States

FAC-SCountTM reagents are a set of laboratory reagents designed for use with flow cytometry instruments. The core function of these reagents is to enable the counting and analysis of cells or particles in a sample. They are intended to provide accurate and reliable cell counting capabilities for various applications in the life sciences and clinical research fields.

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Lab products found in correlation

Tritest trucount monitoring kits, by BD (2 mentions) Facscanto 2 equipment, by BD (2 mentions) Real time hiv 1 amplification matrix, by Abbott (1 mentions) Cba human th1 th2 th17 cytokine kit, by BD (1 mentions) Facscount flow cytometer, by BD (1 mentions) Facscount, by BD (1 mentions)

Close spelling variants of this product

BD FACSTM (2 citations)

4 protocols using fac scount reagents

Comprehensive HIV Diagnostic Protocol

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Suspected HIV infection was confirmed by qualitative detection of the p24 antigen and anti-HIV-1 and anti-HIV-2 IgG antibodies by enzyme immunoassay (Murex AG/AB Combination Diasorin, UK); serological confirmation was performed using a DPP HIV-1/2 rapid immunoblot kit (Bio-Manguinhos, FIOCRUZ) following the manufacturer's recommendations. The CASA DIA samples did not require complementary diagnostic tests since the institution has its own screening panel, which was used for the enrolled patients.
The plasma HIV viral load was quantified by real-time PCR using the Abbott mSample Preparation System RNA Extraction Kit and the Abbott Real-Time HIV-1 Amplification Matrix (ABBOTT, Chicago, Illinois, USA) following the manufacturer's recommendations.
The quantification of CD4 + T (CD45 high CD3 + CD4 + CD8 -) and CD8 + T (CD45 high CD3 + CD4 -CD8 + ) lymphocytes was performed by immunophenotyping and flow cytometry using BD FACSCalibur-4-color equipment and the FAC-SCountTM reagents and TriTEST™/TruCount monitoring kits (BD Biosciences, San Jose, CA, USA), following the manufacturer's recommendations.
The plasma concentrations of the cytokines IL-17 A, IFN-ɣ, TNF, IL-10, IL-6, IL-4 and IL-2 were determined by cytometric bead array (CBA) using BD FACSCanto™ II equipment and the BD™ CBA Human Th1/Th2/Th17 Cytokine Kit (BD Biosciences, San Jose, CA, USA).

Pereira L.M.S., França E.D.S., Costa I.B., Lima I.T., Freire A.B.C., Ramos F.L.P., Monteiro T.A.F., Macedo O., Sousa R.C.M., Freitas F.B., Costa I.B, & Vallinoto A.C.R. (2024). Sociobehavioral Risk Factors and Clinical Implications of Late Presentation Among People Living with HIV in the Brazilian Amazon Region. AIDS and behavior.

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Multiparametric Lymphocyte Profiling and Cytokine Quantification

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The quantification of T helper (CD45^highCD3⁺CD4⁺CD8⁻), T cytotoxic (CD45^highCD3⁺CD4⁻CD8⁺), double positive (DP) (CD45^highCD3⁺CD4⁺CD8⁺), and double negative (DN) (CD45^highCD3⁺CD4⁻CD8⁻) lymphocytes was performed by immunophenotyping and flow cytometry using BD FACSCalibur (4 colors) equipment, FACSCount^TM Reagents, and TriTEST™/TruCount monitoring kits (BD Biosciences, San Jose, CA, USA). We used the BD Multiset™ Software v3.1 software (BD Biosciences, San Jose, CA, USA) already standardized for analysis of lymphocyte populations related to HIV infection.
The plasma concentrations of the cytokines IL-17A, IFN-γ, TNF, IL-10, IL-6, IL-4, and IL-2 were determined with a cytometric bead array (CBA) using BD FACSCanto™ II equipment and a BD^TM CBA Human Th1/Th2/Th17 Cytokine kit (BD Biosciences, San Jose, CA, USA).

Pereira L.M., França E.D., Costa I.B., Lima I.T., Freire A.B., Ramos F.L., Monteiro T.A., Macedo O., Sousa R.C., Freitas F.B., Brasil Costa I, & Vallinoto A.C. (2022). Epstein–Barr Virus (EBV) Genotypes Associated with the Immunopathological Profile of People Living with HIV-1: Immunological Aspects of Primary EBV Infection. Viruses, 14(2), 168.

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QuantiFERON-TB Gold Plus Assay Protocol

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Blood (1 ml) was transferred to each of the four QFT-Plus tubes (nil [background], TB1 [CD4-mediated response], TB2 [CD4- and CD8-mediated response] and mitogen [positive control]), and incubated at 37°C for 16–24 h within 16 h of collection. Following incubation, the samples were returned to ambient room temperature and centrifuged at 2000–3000 relative centrifugal force for 15 min. Plasma was extracted and transferred to a −80°C freezer. Enzyme-linked immunosorbent assay (ELISA) was performed, following re-centrifugation of plasma using standard test kits. The ELISA plate was read at wavelengths of 450 nm and 650 nm. Data were transferred to the QFT-Plus analysis software to calculate results. Positive results were defined according to the manufacturer's threshold (⩾0.35 international unit [IU]/ml); a positive result on either antigen tube, TB1 or TB2, was considered positive. The upper limit for quantifying IFN-γ concentrations was 10 IU/ml. T-lymphocyte estimation (CD4⁺, CD8⁺ and CD3⁺) was performed within 48 h of blood collection using BD FACSCount reagents and a BD FACSCount flow-cytometer (BD, Sparks, MD, USA), according to the manufacturer's specification. The upper limit for CD8 cell count measurement was 2000 cells/μl.

Telisinghe L., Amofa-Sekyi M., Maluzi K., Kaluba-Milimo D., Cheeba-Lengwe M., Chiwele K., Kosloff B., Floyd S., Bailey S.L, & Ayles H. (2017). The sensitivity of the QuantiFERON®-TB Gold Plus assay in Zambian adults with active tuberculosis. The International Journal of Tuberculosis and Lung Disease, 21(6), 690-696.

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Automated Benchtop Cytometry with No-Wash No-Lyse

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The BD FacsCount is a fully automated micro-bead based benchtop cytometer equipped with a green laser for detection of PE and PE.Cy5 labels after no-wash no-lyse sample preparation. We used single-tube BD FacsCount reagents (Ref-No 339010) containing reference beads and monoclonal antibodies conjugated with CD4 PE, CD14 PE-Cy5, and CD15 PE-Cy5 fluorochromes. In short, 50 μL of whole blood was added to the reagent tube using reverse pipetting technique. After 30 minutes incubation in the dark, 50 μL of 5% formaldehyde fixative solution was added before analysis. Gating was done automatically by the built-in BD FacsCount software (Ref-No 339011).

Vogt F., Van den Bergh R., Bernasconi A., Moyo B., Havazvidi L., Bastard M., Flevaud L., Taziwa F., Makondo E, & Mtapuri-Zinyowera S. (2015). Using BD Vacutainer CD4 Stabilization Tubes for Absolute Cluster of Differentiation Type 4 Cell Count Measurement on BD FacsCount and Partec Cyflow Cytometers: A Method Comparison Study from Zimbabwe. PLoS ONE, 10(8), e0136537.

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