Anti-DJ-1 antibodies were purchased from Abcam (Cambridge, US). Anti-p53, Bax, and cleaved caspase-3 antibodies were purchased from Wanleibio (Shenyang, China). FITC-labeled goat anti-rabbit IgG, phenylmethanesulfonyl fluoride (PMSF), and phosphorylated protease inhibitors were purchased from Servicebio (Wuhan, China). Anti-GAPDH and goat anti-rabbit antibodies were purchased from Proteintech (Chicago, IL, USA).
Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Biological Industries (Palestine). 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI), penicillin-streptomycin, Annexin V-FITC Apoptosis Detection kit, and SDS polyacrylamide gel electrophoresis were purchased from Beyotime (Shanghai, China). Hematoxylin and eosin (H&E) and Nissl staining kit were purchased from Sigma (St. Louis, MO, USA). BCA protein assay kits was obtained from Biosharp (Hefei, China). RIPA lysis buffer and nitrocellulose membrane were provided by Millipore (Billerica, MA, USA). The hippocampal neuronal cell line (HT22) and Vero cell line (Vero) were purchased from Procell Life Science (Wuhan, China). TgCtwh3 tachyzoites were kept in Vero cells, respectively, which were stored at -80℃ in our laboratory (Anhui Province Key Laboratory of Microbiology and Parasitology).
Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Biological Industries (Palestine). 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride (DAPI), penicillin-streptomycin, Annexin V-FITC Apoptosis Detection kit, and SDS polyacrylamide gel electrophoresis were purchased from Beyotime (Shanghai, China). Hematoxylin and eosin (H&E) and Nissl staining kit were purchased from Sigma (St. Louis, MO, USA). BCA protein assay kits was obtained from Biosharp (Hefei, China). RIPA lysis buffer and nitrocellulose membrane were provided by Millipore (Billerica, MA, USA). The hippocampal neuronal cell line (HT22) and Vero cell line (Vero) were purchased from Procell Life Science (Wuhan, China). TgCtwh3 tachyzoites were kept in Vero cells, respectively, which were stored at -80℃ in our laboratory (Anhui Province Key Laboratory of Microbiology and Parasitology).