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Recombinant rat egf

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Recombinant rat EGF is a purified protein produced using recombinant DNA technology. It is a growth factor that stimulates cell proliferation and differentiation.

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Lab products found in correlation

Recombinant human fgf21, by Thermo Fisher Scientific (1 mentions) Recombinant human tgf β2, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin bsa, by LGC (1 mentions) Phospho trka, by R&D Systems (1 mentions) Recombinant rat beta ngf, by R&D Systems (1 mentions) Bovine serum albumin (bsa), by Roche (1 mentions)

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Recombinant EGF Rat EGF

2 protocols using recombinant rat egf

1

Growth Factor Supplementation in Rats

Cited 1 time
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TGF-β2, EGF and FGF21 groups were supplemented with recombinant human TGF-β2 (97.3% homology with rat), recombinant rat EGF and recombinant human FGF21 (92% homology with rat) (all from Peprotech, Rocky Hill, NJ, USA). These products were reconstituted in phosphate-buffered solution (PBS, pH 7.2) with 0.1% bovine serum albumin (BSA, SeraCare Life Sciences, Milford, MA, USA), according to the manufacturer’s recommendations. The dose of TGF-β2 was 35 μg/kg/day, which was based on the amount of TGF-β2 found in mid-lactation rat milk and milk intake by pups at 4–14 days of age [13 (link)]. The dose of EGF was 100 μg/kg/day, which was demonstrated to be effective as a treatment in a rat model of NEC [29 (link)]. Finally, the dose of FGF21 was 5 μg/kg/day, an amount that was established in relation to TGF-β2, which was found in a 1:10 ratio FGF21:TGF-β2 [23 (link),30 (link)]. The REF group received a matched volume of the vehicle (1% BSA in PBS) used to dilute the GF.
Torres-Castro P., Grases-Pintó B., Abril-Gil M., Castell M., Rodríguez-Lagunas M.J., Pérez-Cano F.J, & Franch À. (2020). Modulation of the Systemic Immune Response in Suckling Rats by Breast Milk TGF-β2, EGF and FGF21 Supplementation. Nutrients, 12(6), 1888.
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2

Signaling Pathway Activation Assay

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Recombinant rat EGF was obtained from PeproTech (Cat. #400–25) and recombinant rat beta-NGF from R&D Systems (#556-NG-100). Recombinant human NRG1α (Cat. #296-HR) and NRG1β (Cat. #396-HB/CF) were obtained from R&D Systems. All ligands were reconstituted in sterile PBS containing 1 mg/ml bovine serum albumin (BSA) (Roche #03116956001). The following antibodies were used: phospho-p44/42 MAPK (ERK1/2) (pThr202/pTyr204) (E10) (Cell Signaling Technology (CST) #9106), p44/42 MAPK (ERK1/2) (L34F12) (CST #4696), Grb2 (C-23) (Santa Cruz #sc-255) and Grb2 (CST #3972), phospho-EGFR (pTyr1173) (53A5) (CST #4407), phospho-EGFR (pTyr845) (CST #2231), phospho-EGFR (pTyr1086) (CST #2220), phospho-EGFR (pTyr845) (CST #2234), hEGFR (Lab Vision Ab-15: ThermoFisher Sci MS-665-P0), EGFR (CST #2232), phospho-TrkA (pTyr674/675) (C50F3) (CST #4621), phospho-TrkA (pTyr490) (CST #9141), phospho-TrkA (pTyr785) (C67C8) (CST #4168), phospho-TrkA (pTyr785) (R&D #AF5479), TrkA (R&D #AF1056), Rap1A/Rap1B (26B4) (CST #2399), FRS2 (Fisher Sci #PA124685), phospho-ErbB4 (pTyr1242) (4C6) (Santa Cruz #sc81491), phospho-ErbB4 (pTyr1284) (21A9) (CST #4757) and anti-FLAG (CST #2044). The following kinase inhibitors were used: Gö7874 (Calbiochem #365252), erlotinib (CST #5083), and the dynamin GTPase inhibitor Dyngo 4a (Abcam #ab120689).
Kiyatkin A., van Alderwerelt van Rosenburgh I.K., Klein D.E, & Lemmon M.A. (2020). Kinetics of receptor tyrosine kinase activation define ERK signaling dynamics. Science signaling, 13(645), eaaz5267.
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