Cytotoxicity of colistin sulfate and dextrin–colistin conjugates was assessed in HK-2 and NRK-52E cell lines using a multiplexed system that measures cell viability (CellTiter-Blue® (CTB) cell viability assay kit), membrane integrity (CytoTox-One™ homogeneous membrane integrity assay kit): lactate dehydrogenase (LDH release) and caspase activity/apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (link) (see ESI data† for detailed description).
Celltiter blue ctb cell viability assay kit
Manufactured by Promega
Sourced in United States, United Kingdom
The CellTiter-Blue® (CTB) cell viability assay kit is a quantitative colorimetric method used to determine the number of viable cells in a sample. The kit measures the metabolic capacity of cells by assessing their ability to reduce a resazurin-based dye, which changes color in response to cellular metabolic activity.
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2 protocols using celltiter blue ctb cell viability assay kit
1
Cytotoxicity Assays for Colistin
2
Cytotoxicity assessment of currant extracts
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The cytotoxicity of the freeze-dried currant extracts and their identified individual compounds was determined in a range of physiologically relevant concentrations [32, 33] in CaCo-2 cells using the CellTiter-Blue (CTB) cell viability assay kit (Promega-G8081, UK). Cells were seeded in 96-well plates and allowed to get to confluence prior to testing. Concentrations used in our study did not show any significant effect on the metabolic capacity of the cells (Supplemental Fig. 1A &1B).
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