Live/Dead cell imaging was performed using LIVE/DEAD Viability/Cytotoxicity Kit ce(Invitrogen L3224). Half-samples (N = 3) were harvested and immediately incubated in a solution of 2 µM calcein AM and 4 µM ethidium homodimer-1 at room temperature for 30 minutes, washed twice with PBS, and imaged using a Zeiss 710 inverted LSM and LD C-Apochromat 40x/1.1 W Corr M27 objective. A 488 nm laser was split between confocal reflectance and fluorescence. Collagen was visualized as described in 2.3.1 Confocal Imaging Analysis. Live cells were imaged at wavelengths of 499-557 nm. Dead cells were excited with a 561 nm laser, with images collected at 588-735 nm. Representative images were taken across the full length of each construct, with 2-3 images taken in each zone.
Ld c apochromat 40x 1.1 w corr m27 objective
Manufactured by Zeiss
The LD C-Apochromat 40x/1.1 W Corr M27 objective is a high-numerical aperture water immersion objective designed for Carl Zeiss microscopes. It features a magnification of 40x and a numerical aperture of 1.1. The objective is corrected for chromatic aberrations and is suitable for use with water-based samples.
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2 protocols using ld c apochromat 40x 1.1 w corr m27 objective
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Live-Dead Cell Imaging with Confocal Microscopy
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Live-Dead Cell Imaging with Confocal Microscopy
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Live/Dead cell imaging was performed using LIVE/DEAD Viability/Cytotoxicity Kit ce(Invitrogen L3224). Half-samples (N = 3) were harvested and immediately incubated in a solution of 2 µM calcein AM and 4 µM ethidium homodimer-1 at room temperature for 30 minutes, washed twice with PBS, and imaged using a Zeiss 710 inverted LSM and LD C-Apochromat 40x/1.1 W Corr M27 objective. A 488 nm laser was split between confocal reflectance and fluorescence. Collagen was visualized as described in 2.3.1 Confocal Imaging Analysis. Live cells were imaged at wavelengths of 499-557 nm. Dead cells were excited with a 561 nm laser, with images collected at 588-735 nm. Representative images were taken across the full length of each construct, with 2-3 images taken in each zone.
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